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Rho Kinase-related ...
Rho Kinase-related Proteins in Human Vaginal Arteries: An Immunohistochemical and Functional Study
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- Uckert, Stefan (author)
- Hannover Medical School, Division of Surgery, Dept. of Urology & Urological Oncology, Hannover, Germany
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- Waldkirch, Eginhard S (author)
- Hannover Medical School, Division of Surgery, Dept. of Urology & Urological Oncology, Hannover, Germany
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- Kauffels, Wolfgang (author)
- Klinikum Hildesheim GmbH, Dept. of Obstetrics & Gynecology, Hildesheim, Germany
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- Kuczyk, Markus A (author)
- Hannover Medical School, Division of Surgery, Dept. of Urology & Urological Oncology, Hannover, Germany
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- Hedlund, Petter (author)
- Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine,Lund University Hospital, Institute for Laboratory Medicine, Dept. of Clinical & Experimental Pharmacology, Lund, Sweden
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Hannover Medical School, Division of Surgery, Dept of Urology & Urological Oncology, Hannover, Germany Klinikum Hildesheim GmbH, Dept. of Obstetrics & Gynecology, Hildesheim, Germany (creator_code:org_t)
- 2011-10-01
- 2011
- English.
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In: Journal of Sexual Medicine. - : Oxford University Press (OUP). - 1743-6109 .- 1743-6095. ; 8:10, s. 2739-2745
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Abstract
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- The calcium-sensitizing Rho A/Rho kinase pathway has been suggested to play a role in the control of nongenital vascular smooth muscle. Rho-associated kinases (ROKs) cause calcium-independent modulation of smooth muscle contraction, and have been demonstrated in the bladder, prostate, and corpus cavernosum. Until now, it is not known whether ROKs and related proteins play a role in the control of vaginal blood flow. Aim. To investigate by means of functional studies and immunohistochemistry the significance of the Rho pathway in human vaginal arteries. Methods. Vaginal tissue was obtained from five postmenopausal women. Specimens were processed for immunohistochemistry for ROK1, ROK2, RhoA, and RhoGDI. Segments of sub-epithelial vaginal arteries were mounted in a tissue bath. Effects of Y27632 on the concentration-response curves to phenylephrine (Phe) or Phe-precontracted preparations were investigated. Main Outcome Measure. The expression of Rho kinases ROK1, ROK2, and the Rho-associated protein RhoGDI in human vaginal arteries was investigated by means of immunohistochemistry. Tissue bath studies were conducted in order to characterize the effects of the ROK inhibitor Y27632 on isolated vaginal arteries. Results. A meshwork of α-actin immunoreactive arterioles was located in the sub-epithelium of human vaginal specimens. Immunoreactivities for ROK1, ROK2, RhoA, and RhoGDI were expressed in the smooth musculature of these arteries. At 0.1 and 1 µM Y27632, the contraction to Phe (10 µM) was 99 ± 17% and 28 ± 12% that of 124 mM K(+) . In Phe-contracted preparations, Y27632 produced relaxant responses. Conclusions. The activation of alpha(1) -adrenoceptors contracts sub-epithelial human vaginal arteries via ROK-sensitive mechanisms. A role for these signals in the regulation of vaginal blood flow might be considered
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reproduktionsmedicin och gynekologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Obstetrics, Gynaecology and Reproductive Medicine (hsv//eng)
Keyword
- Rho-related Proteins
- Human Vaginal Arteries
- Rho Kinases
- Immunohistochemistry
- Tissue Bath Studies
- Vaginal Blood Flow
- Human Vaginal Arteries; Rho-related Proteins; Rho Kinases; Immunohistochemistry; Tissue Bath Studies; Vaginal Blood Flow
- MEDICINE
Publication and Content Type
- art (subject category)
- ref (subject category)
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