Enteral crude red kidney bean (Phaseolus vulgaris) lectin--phytohemagglutinin--induces maturational changes in the enterocyte membrane proteins of suckling rats.

Kruszewska, DanutaLund University,Lunds universitet,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science (author)

This study aimed to investigate the effect of enterally administered crude red kidney bean (Phaseolus vulgaris) lectin, PHA, on the expression of brush-border membrane vesicle (BBMV) proteins, in particular Na+/H+ exchangers (NHEs), in the small intestine of suckling rats. Gavage of PHA to 14-day-old rats for 3 days resulted in altered protein/glycoprotein patterns as analyzed by SDS-PAGE. Immunoblots demonstrated the appearance of two 71- and 27-kD protein bands indicative for NHE3 - one of the NHE isoforms - and PHA, respectively. PHA treatment also resulted in an augmented uptake of 22Na+ by the BBMV indicating an increase in NHE activity. Overall, the data suggests that enteral PHA exposure may induce maturational changes in enterocyte membrane proteins in young rats. In view of these findings, an investigation into the addition of PHA to infant formulas and weaning diets is warranted.

Kiela, Pawel (author)
Ljungh, ÅsaLund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)mmb-alj (author)
Erlwanger, Kennedy H (author)
Weström, BjörnLund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Functional zoology,Department of Biology,Faculty of Science(Swepub:lu)zoof-bwe (author)
Linderoth, AnnLund University,Lunds universitet,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science(Swepub:lu)zoof-ali (author)
Pierzynowski, StefanLund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Functional zoology,Department of Biology,Faculty of Science(Swepub:lu)zoof-spi (author)
Biologiska institutionenNaturvetenskapliga fakulteten (creator_code:org_t)

By the author/editor: Kruszewska, Danu ...; Kiela, Pawel; Ljungh, Åsa; Erlwanger, Kenne ...; Weström, Björn; Linderoth, Ann; show more...; Pierzynowski, St ...; show less...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Microbiology in ...

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