SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:lup.lub.lu.se:5f3cbf68-eb51-4f8a-8634-a89f5e70701e"
 

Search: onr:"swepub:oai:lup.lub.lu.se:5f3cbf68-eb51-4f8a-8634-a89f5e70701e" > Identification of a...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Wang, Lu (author)

Identification of a novel, recurrent HEY1-NCOA2 fusion in mesenchymal chondrosarcoma based on a genome-wide screen of exon-level expression data

  • Article/chapterEnglish2012

Publisher, publication year, extent ...

  • 2011-10-27
  • Wiley,2012

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:5f3cbf68-eb51-4f8a-8634-a89f5e70701e
  • https://lup.lub.lu.se/record/2278946URI
  • https://doi.org/10.1002/gcc.20937DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • Cancer gene fusions that encode a chimeric protein are often characterized by an intragenic discontinuity in the RNA\expression levels of the exons that are 5' or 3' to the fusion point in one or both of the fusion partners due to differences in the levels of activation of their respective promoters. Based on this, we developed an unbiased, genome-wide bioinformatic screen for gene fusions using Affymetrix Exon array expression data. Using a training set of 46 samples with different known gene fusions, we developed a data analysis pipeline, the Fusion Score (FS) model, to score and rank genes for intragenic changes in expression. In a separate discovery set of 41 tumor samples with possible unknown gene fusions, the FS model generated a list of 552 candidate genes. The transcription factor gene NCOA2 was one of the candidates identified in a mesenchymal chondrosarcoma. A novel HEY1-NCOA2 fusion was identified by 5' RACE, representing an in-frame fusion of HEY1 exon 4 to NCOA2 exon 13. RT-PCR or FISH evidence of this HEY1-NCOA2 fusion was present in all additional mesenchymal chondrosarcomas tested with a definitive histologic diagnosis and adequate material for analysis (n = 9) but was absent in 15 samples of other subtypes of chondrosarcomas. We also identified a NUP107-LGR5 fusion in a dedifferentiated liposarcoma but analysis of 17 additional samples did not confirm it as a recurrent event in this sarcoma type. The novel HEY1-NCOA2 fusion appears to be the defining and diagnostic gene fusion in mesenchymal chondrosarcomas. (C) 2011 Wiley Periodicals, Inc.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Motoi, Toru (author)
  • Khanin, Raya (author)
  • Olshen, Adam (author)
  • Mertens, FredrikLund University,Lunds universitet,Avdelningen för klinisk genetik,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Genetics,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)kgen-fme (author)
  • Bridge, Julia (author)
  • Dal Cin, Paola (author)
  • Antonescu, Cristina R. (author)
  • Singer, Samuel (author)
  • Hameed, Meera (author)
  • Bovee, Judith V. M. G. (author)
  • Hogendoorn, Pancras C. W. (author)
  • Socci, Nicholas (author)
  • Ladanyi, Marc (author)
  • Avdelningen för klinisk genetikInstitutionen för laboratoriemedicin (creator_code:org_t)

Related titles

  • In:Genes, Chromosomes and Cancer: Wiley51:2, s. 127-1391045-2257

Internet link

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view