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Manufacturing of Hu...
Manufacturing of Human Tissues as off-the-Shelf Grafts Programmed to Induce Regeneration
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- Pigeot, Sébastien (author)
- University of Basel,University Hospital Basel
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- Klein, Thibaut (author)
- University of Basel
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- Gullotta, Fabiana (author)
- University of Basel,San Raffaele Hospital
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- Dupard, Steven J. (author)
- Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,WCMM- Wallenberg center för molekylär medicinsk forskning,Reumatologi,Forskargrupper vid Lunds universitet,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine,WCMM-Wallenberg Centre for Molecular Medicine,Rheumatology,Lund University Research Groups
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- Garcia Garcia, Alejandro (author)
- Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,WCMM- Wallenberg center för molekylär medicinsk forskning,Department of Clinical Sciences, Lund,Faculty of Medicine,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,WCMM-Wallenberg Centre for Molecular Medicine
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- García-García, Andres (author)
- University of Basel
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- Prithiviraj, Sujeethkumar (author)
- Lund University,Lunds universitet,Molekylär skelettbiologi,Forskargrupper vid Lunds universitet,Molecular Skeletal Biology,Lund University Research Groups
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- Lorenzo, Pilar (author)
- Lund University,Lunds universitet,WCMM- Wallenberg center för molekylär medicinsk forskning,Medicinska fakulteten,WCMM-Wallenberg Centre for Molecular Medicine,Faculty of Medicine
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- Filippi, Miriam (author)
- University of Basel
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- Jaquiery, Claude (author)
- University of Basel
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- Kouba, Loraine (author)
- University of Basel
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- Asnaghi, M. Adelaide (author)
- University of Basel
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- Raina, Deepak Bushan (author)
- Lund University,Lunds universitet,Ortopedi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Orthopaedics (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Dasen, Boris (author)
- University of Basel
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- Isaksson, Hanna (author)
- Lund University,Lunds universitet,Ortopedi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Orthopaedics (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Önnerfjord, Patrik (author)
- Lund University,Lunds universitet,Reumatologi och molekylär skelettbiologi,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Molekylär skelettbiologi,Forskargrupper vid Lunds universitet,Rheumatology,Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Molecular Skeletal Biology,Lund University Research Groups
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- Tägil, Magnus (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Ortopedi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Orthopaedics (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Bondanza, Attilio (author)
- San Raffaele Hospital
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- Martin, Ivan (author)
- University of Basel,University Hospital Basel
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- Bourgine, Paul E. (author)
- Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,WCMM- Wallenberg center för molekylär medicinsk forskning,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine,WCMM-Wallenberg Centre for Molecular Medicine,University of Basel
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(creator_code:org_t)
- 2021-09-05
- 2021
- English.
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In: Advanced Materials. - : Wiley. - 0935-9648 .- 1521-4095. ; 33:43
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Abstract
Subject headings
Close
- Design criteria for tissue-engineered materials in regenerative medicine include robust biological effectiveness, off-the-shelf availability, and scalable manufacturing under standardized conditions. For bone repair, existing strategies rely on primary autologous cells, associated with unpredictable performance, limited availability and complex logistic. Here, a conceptual shift based on the manufacturing of devitalized human hypertrophic cartilage (HyC), as cell-free material inducing bone formation by recapitulating the developmental process of endochondral ossification, is reported. The strategy relies on a customized human mesenchymal line expressing bone morphogenetic protein-2 (BMP-2), critically required for robust chondrogenesis and concomitant extracellular matrix (ECM) enrichment. Following apoptosis-driven devitalization, lyophilization, and storage, the resulting off-the-shelf cartilage tissue exhibits unprecedented osteoinductive properties, unmatched by synthetic delivery of BMP-2 or by living engineered grafts. Scalability and pre-clinical efficacy are demonstrated by bioreactor-based production and subsequent orthotopic assessment. The findings exemplify the broader paradigm of programming human cell lines as biological factory units to engineer customized ECMs, designed to activate specific regenerative processes.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Keyword
- BMP2
- bone grafts
- endochondral ossification
- extracellular matrices
- regenerative medicine
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Pigeot, Sébastie ...
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Klein, Thibaut
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Gullotta, Fabian ...
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Dupard, Steven J ...
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Garcia Garcia, A ...
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García-García, A ...
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show more...
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Prithiviraj, Suj ...
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Lorenzo, Pilar
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Filippi, Miriam
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Jaquiery, Claude
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Kouba, Loraine
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Asnaghi, M. Adel ...
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Raina, Deepak Bu ...
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Dasen, Boris
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Isaksson, Hanna
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Önnerfjord, Patr ...
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Tägil, Magnus
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Bondanza, Attili ...
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Martin, Ivan
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Bourgine, Paul E ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Biomaterials Sci ...
- Articles in the publication
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Advanced Materia ...
- By the university
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Lund University