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  • Fiorenzano, AlessandroLund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups (author)

Single-cell transcriptomics captures features of human midbrain development and dopamine neuron diversity in brain organoids

  • Article/chapterEnglish2021

Publisher, publication year, extent ...

  • 2021-12-15
  • Springer Science and Business Media LLC,2021

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:626c7cd4-e450-4275-9e2a-707212c358c3
  • https://lup.lub.lu.se/record/626c7cd4-e450-4275-9e2a-707212c358c3URI
  • https://doi.org/10.1038/s41467-021-27464-5DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • Three-dimensional brain organoids have emerged as a valuable model system for studies of human brain development and pathology. Here we establish a midbrain organoid culture system to study the developmental trajectory from pluripotent stem cells to mature dopamine neurons. Using single cell RNA sequencing, we identify the presence of three molecularly distinct subtypes of human dopamine neurons with high similarity to those in developing and adult human midbrain. However, despite significant advancements in the field, the use of brain organoids can be limited by issues of reproducibility and incomplete maturation which was also observed in this study. We therefore designed bioengineered ventral midbrain organoids supported by recombinant spider-silk microfibers functionalized with full-length human laminin. We show that silk organoids reproduce key molecular aspects of dopamine neurogenesis and reduce inter-organoid variability in terms of cell type composition and dopamine neuron formation.

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  • Sozzi, EdoardoLund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups(Swepub:lu)ed5738so (author)
  • Birtele, MarcellaLund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups(Swepub:lu)ma5203bi (author)
  • Kajtez, JankoLund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups(Swepub:lu)ja1386ka (author)
  • Giacomoni, JessicaLund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups(Swepub:lu)je3873gi (author)
  • Nilsson, FredrikLund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups(Swepub:lu)fr5372ni (author)
  • Bruzelius, AndreasLund University,Lunds universitet,Regenerativ neurofysiologi,Forskargrupper vid Lunds universitet,Regenerative Neurophysiology,Lund University Research Groups(Swepub:lu)an1575br (author)
  • Sharma, YogitaLund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups(Swepub:lu)yo4230sh (author)
  • Zhang, YuLund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Human neural utvecklingsbiologi,Developmental and Regenerative Neurobiology,Lund University Research Groups,Human Neural Developmental Biology(Swepub:lu)yu1885zh (author)
  • Mattsson, BengtLund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups(Swepub:lu)neur-bma (author)
  • Emnéus, JennyTechnical University of Denmark(Swepub:lu)je5703em (author)
  • Ottosson, Daniella RylanderLund University,Lunds universitet,Regenerativ neurofysiologi,Forskargrupper vid Lunds universitet,Regenerative Neurophysiology,Lund University Research Groups(Swepub:lu)med-drr (author)
  • Storm, PetterLund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups(Swepub:lu)med-psm (author)
  • Parmar, MalinLund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups(Swepub:lu)mphy-mpa (author)
  • Utvecklings- och regenerativ neurobiologiForskargrupper vid Lunds universitet (creator_code:org_t)

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  • In:Nature Communications: Springer Science and Business Media LLC12:12041-1723

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