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Hydrogels derived f...
Hydrogels derived from decellularized liver tissue support the growth and differentiation of cholangiocyte organoids
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- Willemse, Jorke (author)
- Erasmus University Medical Center
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- van Tienderen, Gilles (author)
- Erasmus University Medical Center
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- van Hengel, Eline (author)
- Erasmus University Medical Center
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- Schurink, Ivo (author)
- Erasmus University Medical Center
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- van der Ven, Diana (author)
- Erasmus University Medical Center
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- Kan, Yik (author)
- Erasmus University Medical Center
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- de Ruiter, Petra (author)
- Erasmus University Medical Center
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- Rosmark, Oskar (author)
- Lund University,Lunds universitet,Lungbiologi,Forskargrupper vid Lunds universitet,Lung Biology,Lund University Research Groups
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- Westergren-Thorsson G, Gunilla (author)
- Lund University,Lunds universitet,Lungbiologi,Forskargrupper vid Lunds universitet,WCMM- Wallenberg center för molekylär medicinsk forskning,Medicinska fakulteten,Lung Biology,Lund University Research Groups,WCMM-Wallenberg Centre for Molecular Medicine,Faculty of Medicine
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Schneeberger, Kerstin (author)
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- van der Eerden, Bram (author)
- Erasmus University Medical Center
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- Roest, Henk (author)
- Erasmus University Medical Center
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Spee, Bart (author)
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- van der Laan, Luc (author)
- Erasmus University Medical Center
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- de Jonge, Jeroen (author)
- Erasmus University Medical Center
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- Verstegen, Monique (author)
- Erasmus University Medical Center
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(creator_code:org_t)
- Elsevier BV, 2022
- 2022
- English.
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In: Biomaterials. - : Elsevier BV. - 0142-9612. ; 284
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Human cholangiocyte organoids are promising for regenerative medicine applications, such as repair of damaged bile ducts. However, organoids are typically cultured in mouse tumor-derived basement membrane extracts (BME), which is poorly defined, highly variable and limits the direct clinical applications of organoids in patients. Extracellular matrix (ECM)-derived hydrogels prepared from decellularized human or porcine livers are attractive alternative culture substrates. Here, the culture and expansion of human cholangiocyte organoids in liver ECM(LECM)-derived hydrogels is described. These hydrogels support proliferation of cholangiocyte organoids and maintain the cholangiocyte-like phenotype. The use of LECM hydrogels does not significantly alter the expression of selected genes or proteins, such as the cholangiocyte marker cytokeratin-7, and no species-specific effect is found between human or porcine LECM hydrogels. Proliferation rates of organoids cultured in LECM hydrogels are lower, but the differentiation capacity of the cholangiocyte organoids towards hepatocyte-like cells is not altered by the presence of tissue-specific ECM components. Moreover, human LECM extracts support the expansion of ICO in a dynamic culture set up without the need for laborious static culture of organoids in hydrogel domes. Liver ECM hydrogels can successfully replace tumor-derived BME and can potentially unlock the full clinical potential of human cholangiocyte organoids.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Extracellular matrix based hydrogel
- Hepatobiliary tissue engineering and regenerative medicine
- Human intrahepatic cholangiocyte organoids
- Liver extracellular matrix extracts
- Whole organ perfusion-based liver decellularization
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Willemse, Jorke
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van Tienderen, G ...
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van Hengel, Elin ...
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Schurink, Ivo
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van der Ven, Dia ...
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Kan, Yik
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show more...
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de Ruiter, Petra
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Rosmark, Oskar
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Westergren-Thors ...
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Schneeberger, Ke ...
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van der Eerden, ...
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Roest, Henk
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Spee, Bart
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van der Laan, Lu ...
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de Jonge, Jeroen
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Verstegen, Moniq ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Biomaterials
- By the university
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Lund University