Search: onr:"swepub:oai:lup.lub.lu.se:632a07b8-3f33-4b73-b2ca-b11c480f3093" > Monitoring the inte...
Fältnamn | Indikatorer | Metadata |
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000 | 05049naa a2200433 4500 | |
001 | oai:lup.lub.lu.se:632a07b8-3f33-4b73-b2ca-b11c480f3093 | |
003 | SwePub | |
008 | 220307s2022 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/632a07b8-3f33-4b73-b2ca-b11c480f30932 URI |
024 | 7 | a https://doi.org/10.1038/s41598-022-06846-92 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Torres-Garcia, Laurau Lund University,Lunds universitet,Experimentell demensforskning,Forskargrupper vid Lunds universitet,Neural plasticitet och reparation,Experimental Dementia Research,Lund University Research Groups,Neural Plasticity and Repair4 aut0 (Swepub:lu)la6204to |
245 | 1 0 | a Monitoring the interactions between alpha-synuclein and Tau in vitro and in vivo using bimolecular fluorescence complementation |
264 | c 2022-02-22 | |
264 | 1 | b Springer Science and Business Media LLC,c 2022 |
520 | a Parkinson’s disease (PD) and Alzheimer’s disease (AD) are characterized by pathological accumulation and aggregation of different amyloidogenic proteins, α-synuclein (aSyn) in PD, and amyloid-β (Aβ) and Tau in AD. Strikingly, few PD and AD patients’ brains exhibit pure pathology with most cases presenting mixed types of protein deposits in the brain. Bimolecular fluorescence complementation (BiFC) is a technique based on the complementation of two halves of a fluorescent protein, which allows direct visualization of protein–protein interactions. In the present study, we assessed the ability of aSyn and Tau to interact with each other. For in vitro evaluation, HEK293 and human neuroblastoma cells were used, while in vivo studies were performed by AAV6 injection in the substantia nigra pars compacta (SNpc) of mice and rats. We observed that the co-expression of aSyn and Tau led to the emergence of fluorescence, reflecting the interaction of the proteins in cell lines, as well as in mouse and rat SNpc. Thus, our data indicates that aSyn and Tau are able to interact with each other in a biologically relevant context, and that the BiFC assay is an effective tool for studying aSyn-Tau interactions in vitro and in different rodent models in vivo. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Neurovetenskaper0 (SwePub)301052 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Neurosciences0 (SwePub)301052 hsv//eng |
700 | 1 | a Domingues, Joana M. P.u Lund University4 aut |
700 | 1 | a Brandi, Edoardou Lund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups4 aut0 (Swepub:lu)ed2621br |
700 | 1 | a Haikal, Carolineu Lund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups4 aut0 (Swepub:lu)ca6087ha |
700 | 1 | a Mudannayake, Janitha M.u Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups4 aut0 (Swepub:lu)ja6032mu |
700 | 1 | a Brás, Inês C.u University Medical Center Göttingen4 aut |
700 | 1 | a Gerhardt, Ellenu University Medical Center Göttingen4 aut |
700 | 1 | a Li, Wenu Lund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups,China Medical University, Shenyang4 aut0 (Swepub:lu)med-wnl |
700 | 1 | a Svanbergsson, Alexanderu Lund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups4 aut0 (Swepub:lu)med-as29 |
700 | 1 | a Outeiro, Tiago F.u University Medical Center Göttingen,Max Planck Institute of Experimental Medicine,University of Newcastle upon Tyne4 aut |
700 | 1 | a Gouras, Gunnar K.u Lund University,Lunds universitet,Experimentell demensforskning,Forskargrupper vid Lunds universitet,Experimental Dementia Research,Lund University Research Groups4 aut0 (Swepub:lu)med-grg |
700 | 1 | a Li, Jia Yiu Lund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups,China Medical University, Shenyang4 aut0 (Swepub:lu)mphy-jli |
710 | 2 | a Experimentell demensforskningb Forskargrupper vid Lunds universitet4 org |
773 | 0 | t Scientific Reportsd : Springer Science and Business Media LLCg 12, s. 1-11q 12<1-11x 2045-2322 |
856 | 4 | u http://dx.doi.org/10.1038/s41598-022-06846-9x freey FULLTEXT |
856 | 4 | u https://www.nature.com/articles/s41598-022-06846-9.pdf |
856 | 4 8 | u https://lup.lub.lu.se/record/632a07b8-3f33-4b73-b2ca-b11c480f3093 |
856 | 4 8 | u https://doi.org/10.1038/s41598-022-06846-9 |
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