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Diverse ultrastruct...
Diverse ultrastructural landscape of atherosclerotic endothelium
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- Kluza, Ewelina (author)
- Eindhoven University of Technology,Academic Medical Center of University of Amsterdam (AMC)
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- Beldman, Thijs J. (author)
- Academic Medical Center of University of Amsterdam (AMC)
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- Shami, Annelie (author)
- Lund University,Lunds universitet,Kardiovaskulär forskning - translationella studier,Forskargrupper vid Lunds universitet,Cardiovascular Research - Translational Studies,Lund University Research Groups
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- Scholl, Edwin R. (author)
- Academic Medical Center of University of Amsterdam (AMC)
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- Malinova, Tsveta S. (author)
- Academic Medical Center of University of Amsterdam (AMC)
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- Grootemaat, Anita E. (author)
- Academic Medical Center of University of Amsterdam (AMC)
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- van der Wel, Nicole N. (author)
- Academic Medical Center of University of Amsterdam (AMC)
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- Gonçalves, Isabel (author)
- Lund University,Lunds universitet,Kardiovaskulär forskning - translationella studier,Forskargrupper vid Lunds universitet,Cardiovascular Research - Translational Studies,Lund University Research Groups,Skåne University Hospital
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- Huveneers, Stephan (author)
- Academic Medical Center of University of Amsterdam (AMC)
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- Mulder, Willem J.M. (author)
- Radboud University Medical Center,Academic Medical Center of University of Amsterdam (AMC),Icahn School of Medicine at Mount Sinai,Eindhoven University of Technology
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- Lutgens, Esther (author)
- University Hospital Munich,Academic Medical Center of University of Amsterdam (AMC),German Centre for Cardiovascular Research
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(creator_code:org_t)
- Elsevier BV, 2021
- 2021
- English 11 s.
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In: Atherosclerosis. - : Elsevier BV. - 0021-9150. ; 339, s. 35-45
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http://dx.doi.org/10... (free)
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http://www.atheroscl...
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Abstract
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- Background and aims: The endothelium plays a major role in atherosclerosis, yet the endothelial plaque surface is a largely uncharted territory. Here we hypothesize that atherosclerosis-driven remodeling of the endothelium is a dynamic process, involving both damaging and regenerative mechanisms. Methods: Using scanning electron microscopy (SEM) and immuno-SEM, we studied endothelial junction ultrastructure, endothelial openings and immune cell-endothelium interactions in eight apoe−/− mice and two human carotid plaques. Results: The surface of early mouse plaques (n = 11) displayed a broad range of morphological alterations, including junctional disruptions and large transcellular endothelial pores with the average diameter between 0.6 and 3 μm. The shoulder region of advanced atherosclerotic lesions (n = 7) had a more aggravated morphology with 8 μm-size paracellular openings at two-fold higher density. In contrast, the central apical surface of advanced plaques, i.e., the plaque body (n = 7), displayed endothelial normalization, as shown by a significantly higher frequency of intact endothelial junctions and a lower incidence of paracellular pores. This normalized endothelial phenotype correlated with low immune cell density (only 5 cells/mm2). The human carotid plaque surface (n = 2) displayed both well-organized and disrupted endothelium with similar features as described above. In addition, they were accompanied by extensive thrombotic areas. Conclusions: Our study unveils the spectrum of endothelial abnormalities associated with the development of atherosclerosis. These were highly abundant in early lesions and in the shoulder region of advanced plaques, while normalized at the advanced plaque's body. Similar endothelial features were observed in human atherosclerotic plaques, underlining the versatility of endothelial transformations in atherosclerosis.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kardiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cardiac and Cardiovascular Systems (hsv//eng)
Keyword
- Atherosclerosis
- Endothelial junctions
- Endothelial permeability
- Endothelium
- Scanning electron microscopy
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Kluza, Ewelina
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Beldman, Thijs J ...
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Shami, Annelie
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Scholl, Edwin R.
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Malinova, Tsveta ...
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Grootemaat, Anit ...
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show more...
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van der Wel, Nic ...
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Gonçalves, Isabe ...
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Huveneers, Steph ...
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Mulder, Willem J ...
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Lutgens, Esther
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cardiac and Card ...
- Articles in the publication
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Atherosclerosis
- By the university
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Lund University