Composite Material Based on Macroporous Polyaniline and Osmium Redox Complex for Biosensor Development

• Here the feasibility of layers based on the conducting polymer polyaniline (PANI) as component of glucose biosensors using glucose oxidase (GOx) as enzyme and [Os(bpy)(2)(4-aminomethylpyridine)Cl]PF6 (OsCmplx) as electrochemical mediator, is evaluated. Particularly, PANI was employed to obtain a nanostructured macroporous material (m-PANI) around polystyrene nanoparticles taken as template and the mediator was co-immobilized during the polymerizing procedure. The GOx biosensor based on OsCmplx modified m-PANI provides a linear response to glucose concentration in the range 5 up to 65 mM with a sensitivity of 3.54 mu A/mM/cm(2) (on a projected geometric area=0.07 cm(2)), an LOD of 0.8 mM and a good precision (%RSD <= 7, n=5); the biosensor is stable showing a decrease of 10% to the value of the sensitivity after 15 days of use and of about 50% after 40 days.

Subject headings

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Keyword

Polyaniline

Macroporous material

Osmium redox complex

Glucose

biosensor

Publication and Content Type

art (subject category)

ref (subject category)