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Deep sequencing of ...
Deep sequencing of uveal melanoma identifies a recurrent mutation in PLCB4.
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Johansson, Peter (author)
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Aoude, Lauren G (author)
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Wadt, Karin (author)
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Glasson, William J (author)
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Warrier, Sunil K (author)
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Hewitt, Alex W (author)
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Kiilgaard, Jens Folke (author)
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Heegaard, Steffen (author)
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Isaacs, Tim (author)
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Franchina, Maria (author)
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- Ingvar, Christian (author)
- Lund University,Lunds universitet,Kirurgi, Lund,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Surgery (Lund),Section V,Department of Clinical Sciences, Lund,Faculty of Medicine
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Vermeulen, Tersia (author)
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Whitehead, Kevin J (author)
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Schmidt, Christopher W (author)
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Palmer, Jane M (author)
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Symmons, Judith (author)
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Gerdes, Anne-Marie (author)
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- Jönsson, Göran B (author)
- Lund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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Hayward, Nicholas K (author)
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(creator_code:org_t)
- 2015-12-14
- 2016
- English 7 s.
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In: Oncotarget. - : Impact Journals, LLC. - 1949-2553. ; 7:4, s. 4624-4631
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Abstract
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- Next generation sequencing of uveal melanoma (UM) samples has identified a number of recurrent oncogenic or loss-of-function mutations in key driver genes including: GNAQ, GNA11, EIF1AX, SF3B1 and BAP1. To search for additional driver mutations in this tumor type we carried out whole-genome or whole-exome sequencing of 28 tumors or primary cell lines. These samples have a low mutation burden, with a mean of 10.6 protein changing mutations per sample (range 0 to 53). As expected for these sun-shielded melanomas the mutation spectrum was not consistent with an ultraviolet radiation signature, instead, a BRCA mutation signature predominated. In addition to mutations in the known UM driver genes, we found a recurrent mutation in PLCB4 (c.G1888T, p.D630Y, NM_000933), which was validated using Sanger sequencing. The identical mutation was also found in published UM sequence data (1 of 56 tumors), supporting its role as a novel driver mutation in UM. PLCB4 p.D630Y mutations are mutually exclusive with mutations in GNA11 and GNAQ, consistent with PLCB4 being the canonical downstream target of the former gene products. Taken together these data suggest that the PLCB4 hotspot mutation is similarly a gain-of-function mutation leading to activation of the same signaling pathway, promoting UM tumorigenesis.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
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- By the author/editor
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Johansson, Peter
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Aoude, Lauren G
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Wadt, Karin
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Glasson, William ...
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Warrier, Sunil K
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Hewitt, Alex W
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show more...
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Kiilgaard, Jens ...
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Heegaard, Steffe ...
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Isaacs, Tim
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Franchina, Maria
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Ingvar, Christia ...
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Vermeulen, Tersi ...
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Whitehead, Kevin ...
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Schmidt, Christo ...
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Palmer, Jane M
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Symmons, Judith
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Gerdes, Anne-Mar ...
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Jönsson, Göran B
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Hayward, Nichola ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
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Oncotarget
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Lund University