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Immunohistochemistr...
Immunohistochemistry subtyping of urothelial carcinoma is feasible in the daily practice
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- Queipo, Francisco Javier (author)
- Hospital Juan Canalejo
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- Unamunzaga, Gorka Muñiz (author)
- Hospital Universitario San Jorge
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- Negro, Begoña Fuertes (author)
- Hospital Universitari de Girona Dr. Josep Trueta
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- Fuertes, Sandra Gracia (author)
- Hospital Universitario San Jorge
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- Cortés, Marina Álvarez (author)
- Hospital Universitario San Jorge
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- Tejedor, Elena Carceller (author)
- Hospital Universitario San Jorge
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- Mañas, Carmen María Bernal (author)
- Hospital Universitario San Jorge
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- Ariño, Arceli Bono (author)
- Hospital Universitario San Jorge
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- Sjödahl, Gottfrid (author)
- Lund University,Lunds universitet,Urologi - blåscancer, Malmö,Forskargrupper vid Lunds universitet,Genomiska analyser av urinblåscancer,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Urinblåsecancer,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Urology - urothelial cancer, Malmö,Lund University Research Groups,Urothelial Cancer Genomics,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Urothelial cancer,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Beorlegui, Carmen (author)
- University of Navarra
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(creator_code:org_t)
- 2022-06-22
- 2022
- English.
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In: Virchows Archiv. - : Springer Science and Business Media LLC. - 0945-6317 .- 1432-2307. ; 481:2, s. 191-200
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Abstract
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- The preferred treatment of choice in muscle-invasive bladder cancer (MIBC) is usually transurethral resection followed by cystectomy, with neoadjuvant chemotherapy being a second option. As the treatment is associated with relevant side effects, a great effort is being made to improve the selection of patients, with molecular subtyping being one of the main strategies. Our aim was to develop an immunohistochemical algorithm for subtyping MIBCs. After a literature review, we have developed a simple algorithm to subtype MIBCs based on their morphology and three common antibodies: GATA3, CK5/6, and p16. We applied it to 113 muscle-invasive carcinomas. The positivity threshold for GATA3 and CK5/6 was 20% with at least moderate intensity, while p16 was 70% with moderate to intense nuclear and cytoplasmic staining. Cases GATA3 + CK5/6 − were considered luminal, while cases GATA3 − CK5/6 + were classified as nonluminal/basal squamous. Luminal p16 + cases were labeled as genomically unstable and luminal p16 − as Uro-like. Cases GATA3 + CK5/6 + with a predominantly basal pattern were labeled luminal, while diffuse cases were labeled nonluminal/basal squamous. All GATA3-CK5/6 − cases were considered nonluminal and were divided into mesenchymal-like or neuroendocrine, depending on the morphology. We were able to classify the 113 cases as: 82 (72.57%) were luminal, being 47 Uro-like (41.59%) and 35 (30.97%) genomically unstable; 31 (27.43%) were nonluminal, being 24 basal/squamous (21.24%), two (1.76%) mesenchymal-like, and five (4.42%) neuroendocrine like. We have achieved a feasible and cost-effective algorithm to subtype MIBCs from morphological features and the use of three common antibodies. Further studies in external cohorts are necessary to validate these results.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Urologi och njurmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Urology and Nephrology (hsv//eng)
Keyword
- Bladder carcinoma
- CK 5/6
- GATA3
- Immunohistochemistry
- Staining
- Subtyping
Publication and Content Type
- art (subject category)
- ref (subject category)
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Queipo, Francisc ...
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Unamunzaga, Gork ...
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Negro, Begoña Fu ...
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Fuertes, Sandra ...
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Cortés, Marina Á ...
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Tejedor, Elena C ...
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Mañas, Carmen Ma ...
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Ariño, Arceli Bo ...
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Sjödahl, Gottfri ...
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Beorlegui, Carme ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Urology and Neph ...
- Articles in the publication
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Virchows Archiv
- By the university
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Lund University