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Rapid Single-Nucleo...
Rapid Single-Nucleotide Polymorphism Detection of Cytochrome P450 (CYP2C9) and Vitamin K Epoxide Reductase (VKORC1) Genes for the Warfarin Dose Adjustment by the SMart-Amplification Process Version 2
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Aomori, Tohru (author)
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Yamamoto, Koujirou (author)
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Oguchi-Katayama, Atsuko (author)
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Kawai, Yuki (author)
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Ishidao, Takefumi (author)
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Mitani, Yasumasa (author)
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Kogo, Yasushi (author)
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Lezhava, Alexander (author)
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Fujita, Yukiyoshi (author)
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Obayashi, Kyoko (author)
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Nakamura, Katsunori (author)
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- Kohnke, Hugo (author)
- Uppsala universitet,Klinisk farmakologi
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- Wadelius, Mia (author)
- Uppsala universitet,Klinisk farmakogenomik och osteoporos
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- Ekström, Lena (author)
- Karolinska Institutet
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- Skogastierna, Cristine (author)
- Karolinska Institutet
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- Rane, Anders (author)
- Karolinska Institutet
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Kurabayashi, Masahiko (author)
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Murakami, Masami (author)
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Cizdziel, Paul E. (author)
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Hayashizaki, Yoshihide (author)
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Horiuchi, Ryuya (author)
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(creator_code:org_t)
- 2009-04-01
- 2009
- English.
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In: Clinical Chemistry. - : Oxford University Press (OUP). - 0009-9147 .- 1530-8561. ; 55:4, s. 804-812
- Related links:
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http://clinchem.aacc...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- BACKGROUND: Polymorphisms of the CYP2C9 (cytochrome P450, family 2, subfamily C, polypeptide 9) gene (CYP2C9*2, CYP2C9*3) and the VKORC1 (vitamin K epoxide reductase complex, subunit 1) gene (-1639G>A) greatly impact the maintenance dose for the drug warfarin. Prescreening patients for their genotypes before prescribing the drug facilitates a faster individualized determination of the proper maintenance dose, minimizing the risk for adverse reaction and reoccurrence of thromboembolic episodes. With current methodologies, therapy can be delayed by several hours to 1 day if genotyping is to determine the loading dose. A simpler and more rapid genotyping method is required. METHODS: We developed a single-nucleotide polymorphism (SNP)-detection assay based on the SMart Amplification Process version 2 (SMAP 2) to analyze CYP2C9*2, CYP2C9*3, and VKORC1 -1639G>A polymorphisms. Blood from consenting participants was used directly in a closed-tube real-time assay without DNA purification to obtain results within 1 h of blood collection. RESULTS: We analyzed 125 blood samples by both SMAP 2 and PCR-RFLP methods. The results showed perfect concordance. CONCLUSIONS: The results validate the accuracy of the SMAP 2 for determination of SNPs critical to personalized warfarin therapy. SMAP 2 offers speed, simplicity of sample preparation, the convenience of isothermal amplification, and assay-design flexibility, which are significant advantages over conventional genotyping technologies. In this example and other clinical scenarios in which genetic testing is required for immediate and better-informed therapeutic decisions, SMAP 2-based diagnostics have key advantages.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Keyword
- MEDICINE
- MEDICIN
- Clinical pharmacology
- Klinisk farmakologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Aomori, Tohru
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Yamamoto, Koujir ...
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Oguchi-Katayama, ...
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Kawai, Yuki
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Ishidao, Takefum ...
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Mitani, Yasumasa
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show more...
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Kogo, Yasushi
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Lezhava, Alexand ...
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Fujita, Yukiyosh ...
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Obayashi, Kyoko
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Nakamura, Katsun ...
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Kohnke, Hugo
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Wadelius, Mia
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Ekström, Lena
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Skogastierna, Cr ...
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Rane, Anders
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Kurabayashi, Mas ...
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Murakami, Masami
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Cizdziel, Paul E ...
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Hayashizaki, Yos ...
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Horiuchi, Ryuya
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Pharmacology and ...
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Clinical Chemist ...
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Uppsala University
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Karolinska Institutet