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Effect of murine st...
Effect of murine strain on metabolic pathways of glucose production after brief or prolonged fasting
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Burgess, SC (author)
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Jeffrey, FMH (author)
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Storey, C (author)
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Milde, A (author)
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Hausler, N (author)
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Merritt, ME (author)
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- Mulder, Hindrik (author)
- Lund University,Lunds universitet,Diabetes - molekylär metabolism,Forskargrupper vid Lunds universitet,Diabetes - Molecular Metabolism,Lund University Research Groups
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- Holm, Cecilia (author)
- Lund University,Lunds universitet,Molekylär endokrinologi,Forskargrupper vid Lunds universitet,Molecular Endocrinology,Lund University Research Groups
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Sherry, AD (author)
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Malloy, CR (author)
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(creator_code:org_t)
- American Physiological Society, 2005
- 2005
- English.
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In: American Journal of Physiology: Endocrinology and Metabolism. - : American Physiological Society. - 1522-1555 .- 0193-1849. ; 289:1, s. 53-61
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Abstract
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- Background strain is known to influence the way a genetic manipulation affects mouse phenotypes. Despite data that demonstrate variations in the primary phenotype of basic inbred strains of mice, there is limited data available about specific metabolic fluxes in vivo that may be responsible for the differences in strain phenotypes. In this study, a simple stable isotope tracer/NMR spectroscopic protocol has been used to compare metabolic fluxes in ICR, FVB/N (FVB), C57BL/6J (B6), and 129S1/SvImJ (129) mouse strains. After a short-term fast in these mice, there were no detectable differences in the pathway fluxes that contribute to glucose synthesis. However, after a 24-h fast, B6 mice retain some residual glycogenolysis compared with other strains. FVB mice also had a 30% higher in vivo phosphoenolpyruvate carboxykinase flux and total glucose production from the level of the TCA cycle compared with B6 and 129 strains, while total body glucose production in the 129 strain was similar to 30% lower than in either FVB or B6 mice. These data indicate that there are inherent differences in several pathways involving glucose metabolism of inbred strains of mice that may contribute to a phenotype after genetic manipulation in these animals. The techniques used here are amenable to use as a secondary or tertiary tool for studying mouse models with disruptions of intermediary metabolism.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Keyword
- stable isotope
- metabolic flux
- phosphoenolpyruvate carboxykinase
- nuclear magnetic resonance
- tricarboxylic acid cycle
- tracers
- deuterium
- mouse phenotype
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Burgess, SC
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Jeffrey, FMH
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Storey, C
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Milde, A
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Hausler, N
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Merritt, ME
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show more...
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Mulder, Hindrik
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Holm, Cecilia
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Sherry, AD
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Malloy, CR
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Physiology
- Articles in the publication
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American Journal ...
- By the university
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Lund University