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Label-free acoustophoretic enrichment of mononuclear cells from blood
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- Urbansky, Anke (author)
- Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Acoustofluidics group,Forskargrupper vid Lunds universitet,Other operations, LTH,Faculty of Engineering, LTH,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Lund University Research Groups
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- Ohlsson, Pelle (author)
- Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Acoustofluidics group,Forskargrupper vid Lunds universitet,Other operations, LTH,Faculty of Engineering, LTH,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Lund University Research Groups,AcouSort AB
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- Lenshof, Andreas (author)
- Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Acoustofluidics group,Forskargrupper vid Lunds universitet,Other operations, LTH,Faculty of Engineering, LTH,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Lund University Research Groups
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- (creator_code:org_t)
- 2017
- 2017
- English 2 s.
- In: MicroTAS 2017 : Savannah, Georgia, USA - Savannah, Georgia, USA. - 1556-5904. - 9780692941836 ; 2017, s. 1309-1310
- Conference paper (peer-reviewed)
Abstract
Subject headings
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- We are reporting an efficient, label-free and continuous separation of mononuclear cells (MNC), from blood using microchip acoustophoresis. In standard PBS buffer, MNCs and red blood cells (RBC) display overlapping acoustophoretic mobilities which compromise separation of these cell types from each other. In this paper we capitalize on the fact that MNC and RBC display different acoustophysical properties. By optimizing the buffer conditions and thereby changing the acoustic contrast factor, and hence the acoustophoretic mobility of the cells, a 2800-fold enrichment of MNCs vs. RBCs with MNC recoveries up to 88% was accomplished.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- Acoustophoresis
- Cell Separation
- Label-free
- Mononuclear Cells
- Red Blood Cells
Publication and Content Type
- kon (subject category)
- ref (subject category)
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- MicroTAS 2017 : Savannah, Georgia, USA - Savannah, Georgia, USA (Search for host publication in LIBRIS)
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- Urbansky, Anke
- Ohlsson, Pelle
- Lenshof, Andreas
- Scheding, Stefan
- Laurell, Thomas
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