Search: onr:"swepub:oai:lup.lub.lu.se:6cea802e-cf81-425b-9d54-fdf1351caea5" >
Arterial Blood Pres...
Arterial Blood Pressure Induces Transient C4b-Binding Protein in Human Saphenous Vein Grafts
-
- Kupreishvili, Koba (author)
- Amsterdam UMC - Vrije Universiteit Amsterdam
-
- Meischl, Christof (author)
- Amsterdam UMC - Vrije Universiteit Amsterdam
-
- Vonk, Alexander B. A. (author)
- Amsterdam UMC - Vrije Universiteit Amsterdam
-
show more...
-
- Stooker, Wim (author)
- Onze Lieve Vrouwe Gasthuis
-
- Eijsman, Leon (author)
- Amsterdam UMC - Vrije Universiteit Amsterdam
-
- Blom, Anna M. (author)
- Lund University,Lunds universitet,Institutionen för translationell medicin,Medicinska fakulteten,Department of Translational Medicine,Faculty of Medicine
-
- Quax, Paul H A (author)
- Leiden University Medical Centre
-
- van Hinsbergh, Victor W M (author)
- Amsterdam UMC - Vrije Universiteit Amsterdam
-
- Niessen, Hans W M (author)
- Amsterdam UMC - Vrije Universiteit Amsterdam
-
- Krijnen, Paul A. J. (author)
- Amsterdam UMC - Vrije Universiteit Amsterdam
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2017
- 2017
- English.
-
In: Annals of Vascular Surgery. - : Elsevier BV. - 0890-5096. ; 41, s. 259-264
- Related links:
-
http://dx.doi.org/10...
-
show more...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Background: Complement is an important mediator in arterial blood pressure-induced vein graft failure. Previously, we noted activation of cell protective mechanisms in human saphenous veins too. Here we have analyzed whether C4b-binding protein (C4bp), an endogenous complement inhibitor, is present in the vein wall. Methods: Human saphenous vein segments obtained from patients undergoing coronary artery bypass grafting (n = 55) were perfused in vitro at arterial blood pressure with either autologous blood for 1, 2, 4, or 6 hr or with autologous blood supplemented with reactive oxygen species scavenger N-acetylcysteine. The segments were subsequently analyzed quantitatively for presence of C4bp and complement activation product C3d using immunohistochemistry. Results: Perfusion induced deposition of C3d and C4bp within the media of the vessel wall, which increased reproducibly and significantly over a period of 4 hr up to 3.8% for C3d and 81% for C4bp of the total vessel area. Remarkably after 6 hr of perfusion, the C3d-positive area decreased significantly to 1.3% and the C4bp-positive area to 19% of the total area of the vein. The areas positive for both C4bp and C3d were increased in the presence of N-acetylcysteine. Conclusions: Exposure to arterial blood pressure leads to a transient presence of C4bp in the vein wall. This may be part of a cell-protective mechanism to counteract arterial blood pressure-induced cellular stress and inflammation in grafted veins.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kardiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cardiac and Cardiovascular Systems (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database
- By the author/editor
-
Kupreishvili, Ko ...
-
Meischl, Christo ...
-
Vonk, Alexander ...
-
Stooker, Wim
-
Eijsman, Leon
-
Blom, Anna M.
-
show more...
-
Quax, Paul H A
-
van Hinsbergh, V ...
-
Niessen, Hans W ...
-
Krijnen, Paul A. ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Cardiac and Card ...
- Articles in the publication
-
Annals of Vascul ...
- By the university
-
Lund University