Expression and Distribution of Cyclic AMP- and Cyclic GMP-Binding Protein Kinases in the Human Vagina-An Immunohistochemical Study

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Expression and Distribution of Cyclic AMP- and Cyclic GMP-Binding Protein Kinases in the Human Vagina-An Immunohistochemical Study

Ueckert, Stefan (author): Division of Surgery, Department of Urology and Uro-Oncology, Hannover Medical School, Hannover, Germany

Waldkirch, Eginhard S. (author): Division of Surgery, Department of Urology and Uro-Oncology, Hannover Medical School, Hannover, Germany

Albrecht, Knut (author): Division of Surgery, Department of Urology and Uro-Oncology, Hannover Medical School, Hannover, Germany

Sonnenberg, Julia (author): Division of Surgery, Department of Urology and Uro-Oncology, Hannover Medical School, Hannover, Germany

Langnaese, Kristina (author): Faculty of Medicine, Institute for Biochemistry and Cell Biology, Otto-von-Guericke University, Magdeburg, Germany

Richter, Karin (author): Faculty of Medicine, Institute for Biochemistry and Cell Biology, Otto-von-Guericke University, Magdeburg, Germany

Hedlund, Petter (author): Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine,Faculty of Medicine, University Hospital, Department of Clinical and Experimental Pharmacology, Lund University, Lund, Sweden

Kuczyk, Markus A. (author): Division of Surgery, Department of Urology and Uro-Oncology, Hannover Medical School, Hannover, Germany

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Oxford University Press (OUP), 2010
2010
English.
In: Journal of Sexual Medicine. - : Oxford University Press (OUP). - 1743-6109 .- 1743-6095. ; 7:2, s. 888-895

Related links:: http://dx.doi.org/10...; show more...; https://lup.lub.lu.s...; https://doi.org/10.1...; https://urn.kb.se/re...; show less...

Journal article (peer-reviewed)

Abstract Subject headings

Introduction. In contrast to research findings describing the localization of nitric oxide synthases (NOS), guanylyl cyclases, and cyclic adenosine monophosphate (cAMP)- and cyclic guanosine monophosphate (cGMP)-degrading phosphodiesterase isoenzymes in the human vagina, the distribution of proteins known as major targets for cyclic nucleotides has not yet been evaluated. cAMP- and cGMP-dependent protein kinases (cAK, cGKI) have been identified as important receptors for cyclic nucleotides downstream the signaling cascades. Aim. To investigate, by means of immunohistochemistry, the expression of cAK and cGKI in relation to endothelial NOS (eNOS), vasoactive intestinal polypeptide (VIP), and protein gene product 9.5 (PGP 9.5) in the human vagina. Main Outcome Measures. Expression and distribution of cAK and cGKI(alpha,beta) in relation to eNOS, VIP, and PGP 9.5 in human vaginal tissue. Methods. Immunohistochemical techniques were applied to sections of human vaginal full wall specimens in order to evaluate the presence of cAK and cGKI(alpha,beta) in relation to VIP, PGP 9.5, and eNOS, respectively. Western blot analyses were conducted using cytosolic supernatants of homogenized specimens of the vaginal wall and epithelium. Results. Immunostaining specific for cGKI beta was observed in vascular and nonvascular smooth muscle of the vagina. In the endothelial layer, cGKI beta was found colocalized with eNOS. In contrast, no signals indicating cGKI alpha were registered. cAK-positive subepithelial vessels were found to be innervated by a dense meshwork of PGP-containing varicose nerve fibers, some of which presented expression of VIP. The expression of cAK and cGKI beta was confirmed by Western blotting. Conclusions. Our results demonstrate the expression of cAK and cGKI beta in the human vagina. The colocalization with VIP and eNOS underlines the significance of both the cAMP and GMP pathway in the control of human vaginal vascular and nonvascular smooth muscle. Uckert S, Waldkirch ES, Albrecht K, Sonnenberg J, Langnase K, Richter K, Hedlund P, and Kuczyk MA. Expression and distribution of cyclic AMP- and cyclic GMP-binding protein kinases in the human vagina-An immunohistochemical study.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reproduktionsmedicin och gynekologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Obstetrics, Gynaecology and Reproductive Medicine (hsv//eng)

Keyword

Immunohistochemistry
Cyclic GMP
Cyclic AMP
Vaginal Physiology
Protein Kinases
Vaginal Physiology; Protein Kinases; Cyclic AMP; Cyclic GMP; Immunohistochemistry
MEDICINE

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By the author/editor: Ueckert, Stefan; Waldkirch, Eginh ...; Albrecht, Knut; Sonnenberg, Juli ...; Langnaese, Krist ...; Richter, Karin; show more...; Hedlund, Petter; Kuczyk, Markus A ...; show less...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Obstetrics Gynae ...

Articles in the publication: Journal of Sexua ...

By the university: Lund University; Linköping University

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