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The serine protease...
The serine protease HtrA plays a key role in heat-induced dispersal of pneumococcal biofilms
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- Chao, Yashuan (author)
- Lund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Experimentell infektionsmedicin, Malmö,Forskargrupper vid Lunds universitet,Immunmodulerande effekter av blodplättar vid inflammation och infektion,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Experimental Infection Medicine, Malmö,Lund University Research Groups,Immunomodulatory effects of platelets during inflammation and infection
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- Bergenfelz, Caroline (author)
- Lund University,Lunds universitet,Experimentell infektionsmedicin, Malmö,Forskargrupper vid Lunds universitet,Experimental Infection Medicine, Malmö,Lund University Research Groups
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- Sun, Renhua (author)
- Karolinska Institutet
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- Han, Xiao (author)
- Karolinska Institutet
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- Achour, Adnane (author)
- Karolinska Institutet
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- Hakansson, Anders P (author)
- Lund University,Lunds universitet,Experimentell infektionsmedicin, Malmö,Forskargrupper vid Lunds universitet,SEBRA Sepsis and Bacterial Resistance Alliance,Experimental Infection Medicine, Malmö,Lund University Research Groups
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(creator_code:org_t)
- 2020-12-31
- 2020
- English 11 s.
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In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 10:1
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Abstract
Subject headings
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- Streptococcus pneumoniae (the pneumococcus) colonizes the human nasopharynx by forming multicellular biofilms. Due to the high level of asymptomatic carriage, transition to infections, such as otitis media, pneumonia, sepsis, and meningitis, occurs often enough that the pneumococcus remains a major cause of disease and death globally. Virus infection and virus-induced responses, such as increased temperature (fever), trigger release of virulent bacteria from colonizing biofilms. The exact mechanisms involved in pneumococcal egress during biofilm dispersal remain unknown, although we hypothesize that disruption of the biofilm matrix encasing the bacteria is necessary. Here, we utilized established in vitro biofilm dispersal models to investigate the involvement of proteases in bacterial egress from pneumococcal biofilms. We demonstrate the importance of protease activity, both through increased bacterial release following addition of proteases and reduced heat-induced biofilm dispersal in the presence of protease inhibitors. We identify a key role for the surface-exposed serine protease HtrA, but not PrtA, in heat-induced biofilm dispersal. Bacterial release from htrA-negative biofilms was significantly reduced compared to wild-type isogenic strains but was restored and increased above wild-type levels following addition of recombinant HtrA. Understanding the specific mechanisms involved in bacterial egress may provide novel targets for future strategies aimed to specifically interfere with disease progression without disturbing nasopharyngeal biofilm colonization.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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