onr:"swepub:oai:lup.lub.lu.se:72626f22-9a99-47f9-b7f8-52106152e979"
Search: onr:"swepub:oai:lup.lub.lu.se:72626f22-9a99-47f9-b7f8-52106152e979" > A new luminescence ...
- 1 of 1
- Previous record
- Next record
- To hitlist
A new luminescence assay for autoantibodies to mammalian cell-prepared insulinoma-associated protein 2
- Burbelo, Peter D. (author)
- Hirai, Hiroki (author)
- Leahy, Hannah (author)
- show more...
- Lernmark, Ake (author)
- Iadarola, Michael J. (author)
- Notkins, Abner Louis (author)
- show less...
- (creator_code:org_t)
- American Diabetes Association, 2008
- 2008
- English.
- In: Diabetes Care. - : American Diabetes Association. - 1935-5548 .- 0149-5992. ; 31:9, s. 1824-1826
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- OBJECTIVE - Insulinoma-associated protein 2 (IA-2) is a major autoantigen in type I diabetes, and IA-2 autoantibodies are routinely detected by a liquid-phase radioimmunoprecipitation assay. The present experiments were initiated to develop a new assay that does not require the use of radioisotopes or autoantigens prepared in bacteria or by in vitro transcription/translation. RESEARCH DESIGN AND METHODS - IA-2 luciferase fusion protein was expressed in mammalian cells and assayed for autoantibodies by liquid-phase luciferase immunoprecipitation. RESULTS - Our study showed that there was no significant difference between the luciferase immunoprecipitation and the radioimmunoprecipitation assays in sensitivity and specificity, and comparison of the two assays revealed a high correlation coefficient (R-2 = 0.805). CONCLUSIONS - The luciferase system offers a robust, inexpensive, nonradioactive method for the detection of autoantibodies to mammalian cell-prepared IA-2 and could be of practical value at the clinical level.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
- Diabetes Care (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Burbelo, Peter D ...
- Hirai, Hiroki
- Leahy, Hannah
- Lernmark, Ake
- Ivarsson, Sten
- Iadarola, Michae ...
- show more...
- Notkins, Abner L ...
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Clinical Medicin ...
- and Endocrinology an ...
- Articles in the publication
- Diabetes Care
- By the university
- Lund University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
