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A new luminescence ...
A new luminescence assay for autoantibodies to mammalian cell-prepared insulinoma-associated protein 2
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Burbelo, Peter D. (author)
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Hirai, Hiroki (author)
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Leahy, Hannah (author)
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Lernmark, Ake (author)
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- Ivarsson, Sten (author)
- Lund University,Lunds universitet,Pediatrisk endokrinologi,Forskargrupper vid Lunds universitet,Paediatric Endocrinology,Lund University Research Groups
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Iadarola, Michael J. (author)
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Notkins, Abner Louis (author)
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(creator_code:org_t)
- American Diabetes Association, 2008
- 2008
- English.
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In: Diabetes Care. - : American Diabetes Association. - 1935-5548 .- 0149-5992. ; 31:9, s. 1824-1826
- Related links:
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http://dx.doi.org/10... (free)
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http://care.diabetes...
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https://lup.lub.lu.s...
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https://doi.org/10.2...
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Abstract
Subject headings
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- OBJECTIVE - Insulinoma-associated protein 2 (IA-2) is a major autoantigen in type I diabetes, and IA-2 autoantibodies are routinely detected by a liquid-phase radioimmunoprecipitation assay. The present experiments were initiated to develop a new assay that does not require the use of radioisotopes or autoantigens prepared in bacteria or by in vitro transcription/translation. RESEARCH DESIGN AND METHODS - IA-2 luciferase fusion protein was expressed in mammalian cells and assayed for autoantibodies by liquid-phase luciferase immunoprecipitation. RESULTS - Our study showed that there was no significant difference between the luciferase immunoprecipitation and the radioimmunoprecipitation assays in sensitivity and specificity, and comparison of the two assays revealed a high correlation coefficient (R-2 = 0.805). CONCLUSIONS - The luciferase system offers a robust, inexpensive, nonradioactive method for the detection of autoantibodies to mammalian cell-prepared IA-2 and could be of practical value at the clinical level.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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