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Preliminary Researc...
Preliminary Research : Application of Non-Invasive Measure of Cytochrome c Oxidase Redox States and Mitochondrial Function in a Porcine Model of Carbon Monoxide Poisoning
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- Lewis, Alistair T. (author)
- University of Pennsylvania,The Children's Hospital of Philadelphia
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- Forti, Rodrigo M. (author)
- The Children's Hospital of Philadelphia
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- Alomaja, Oladunni (author)
- University of Pennsylvania
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- Mesaros, Clementina (author)
- University of Pennsylvania
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- Piel, Sarah (author)
- The Children's Hospital of Philadelphia
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- Greenwood, John C. (author)
- University of Pennsylvania
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- Talebi, Fatima M. (author)
- University of Pennsylvania
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- Mavroudis, Constantine D. (author)
- The Children's Hospital of Philadelphia
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- Kelly, Matthew (author)
- University of Alabama
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- Kao, Shih Han (author)
- The Children's Hospital of Philadelphia
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- Shofer, Frances S. (author)
- University of Pennsylvania
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- Ehinger, Johannes K. (author)
- Lund University,Lunds universitet,Öron-, näs- och halssjukdomar, Lund,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Otorhinolaryngology (Lund),Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,Skåne University Hospital
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- Kilbaugh, Todd J. (author)
- The Children's Hospital of Philadelphia
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- Baker, Wesley B. (author)
- The Children's Hospital of Philadelphia
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- Jang, David H. (author)
- University of Pennsylvania,The Children's Hospital of Philadelphia
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(creator_code:org_t)
- 2022-04-28
- 2022
- English 9 s.
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In: Journal of Medical Toxicology. - : Springer Science and Business Media LLC. - 1556-9039 .- 1937-6995. ; 18:3, s. 214-222
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Abstract
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- Introduction: Carbon monoxide (CO) is a colorless and odorless gas that is a leading cause of environmental poisoning in the USA with substantial mortality and morbidity. The mechanism of CO poisoning is complex and includes hypoxia, inflammation, and leukocyte sequestration in brain microvessel segments leading to increased reactive oxygen species. Another important pathway is the effects of CO on the mitochondria, specifically at cytochrome c oxidase, also known as Complex IV (CIV). The purpose of this ongoing study is the preliminary development of a porcine model of CO poisoning for investigation of alterations in brain mitochondrial physiology. Methods: Four pigs (10 kg) were divided into two groups: Sham (n = 2) and CO (n = 2). Administration of a dose of CO at 2000 ppm to the CO group over 120 minutes followed by 30 minutes of re-oxygenation at room air. The control group received room air for 150 minutes. Non-invasive optical monitoring was used to measure CIV redox states. Cerebral microdialysis was performed to obtain semi real-time measurements of cerebral metabolic status. At the end of the exposure, fresh brain tissue (cortical and hippocampal) was immediately harvested to measure mitochondrial respiration. Snap frozen cortical tissue was also used for ATP concentrations and western blotting. Results: While a preliminary ongoing study, animals in the CO group showed possible early decreases in brain mitochondrial respiration, citrate synthase density, CIV redox changes measured with optics, and an increase in the lactate-to-pyruvate ratio. Conclusions: There is a possible observable phenotype highlighting the important role of mitochondrial function in the injury of CO poisoning.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Anestesi och intensivvård (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Anesthesiology and Intensive Care (hsv//eng)
Keyword
- Basic science
- Biomarker
- Carbon monoxide
- Mitochondria
- Optics
Publication and Content Type
- art (subject category)
- ref (subject category)
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Lewis, Alistair ...
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Forti, Rodrigo M ...
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Alomaja, Oladunn ...
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Mesaros, Clement ...
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Piel, Sarah
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Greenwood, John ...
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show more...
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Talebi, Fatima M ...
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Mavroudis, Const ...
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Kelly, Matthew
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Kao, Shih Han
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Shofer, Frances ...
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Ehinger, Johanne ...
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Kilbaugh, Todd J ...
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Baker, Wesley B.
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Jang, David H.
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Anesthesiology a ...
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Lund University