Resveratrol suppressed lps-induced cox-2 VIA miR-146a-5p inhibition in raw246.7 cells

Dao, Trong Tuan (author): National Institute of Medicinal Materials, Vietnam

Hien Tran, Thi (author): Lund University,Lunds universitet,Molekylär kärlfysiologi,Forskargrupper vid Lunds universitet,Molecular Vascular Physiology,Lund University Research Groups,Thai Binh University of Medicine and Pharmacy

Long, Phung Thanh (author): National Institute of Medicinal Materials, Vietnam

Thu, Nguyen Bich (author): National Institute of Medicinal Materials, Vietnam

Khoi, Nguyen Minh (author): National Institute of Medicinal Materials, Vietnam

Ngoc, Tran Minh (author): National Institute of Medicinal Materials, Vietnam

(creator_code:org_t)

Abstract Subject headings

Trans-resveratrol (Res) is a well-known natural stilbene frequently found in grapes which have been reported to possess antioxidant, anti-cancer activities and inhibited COX-2 expression. MicroRNAs (miRNAs) are short endogenous non-coding RNAs involved in the regulation of mRNA stability and protein synthesis. In our research, resveratrol isolated from Vitis heyneana Roem. & Schult Vitis heyneana was observed to suppress lipopolysaccharides (LPS)-induced COX-2 expression in Raw264.7 cells in a dose dependent manner. Using qPCR it was revealed that LPS induced the expression of miR-25, miR- 125a, miR-125b, miR-146a-5p, miR-146a-3p and miR-455. However, we only observed miR-146a-5p expression significantly decreased in resveratrol compared to untreated-control group. In addition, resveratrol abrogated the effect of miR-146a-5p mimic induced-COX-2 expression in Raw264.7 cells. Taken together, this study demonstrated for the first time the involvement of miR-146a-5p in resveratrol inhibited LPS-induced COX-2 expression in Raw264.7 cells.

By the author/editor: Dao, Trong Tuan; Ha, Do Thi; Hien Tran, Thi; Binh, Bui Thi; Phuong, Tran Thi; Long, Phung Than ...; show more...; Thu, Nguyen Bich; Que, Do Thi Nguy ...; Khoi, Nguyen Min ...; Dung, Le Viet; Ngoc, Tran Minh; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Pharmacology and ...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.