Resveratrol suppressed lps-induced cox-2 VIA miR-146a-5p inhibition in raw246.7 cells

Trans-resveratrol (Res) is a well-known natural stilbene frequently found in grapes which have been reported to possess antioxidant, anti-cancer activities and inhibited COX-2 expression. MicroRNAs (miRNAs) are short endogenous non-coding RNAs involved in the regulation of mRNA stability and protein synthesis. In our research, resveratrol isolated from Vitis heyneana Roem. & Schult Vitis heyneana was observed to suppress lipopolysaccharides (LPS)-induced COX-2 expression in Raw264.7 cells in a dose dependent manner. Using qPCR it was revealed that LPS induced the expression of miR-25, miR- 125a, miR-125b, miR-146a-5p, miR-146a-3p and miR-455. However, we only observed miR-146a-5p expression significantly decreased in resveratrol compared to untreated-control group. In addition, resveratrol abrogated the effect of miR-146a-5p mimic induced-COX-2 expression in Raw264.7 cells. Taken together, this study demonstrated for the first time the involvement of miR-146a-5p in resveratrol inhibited LPS-induced COX-2 expression in Raw264.7 cells.

Ha, Do ThiNational Institute of Medicinal Materials, Vietnam (author)
Hien Tran, ThiLund University,Lunds universitet,Molekylär kärlfysiologi,Forskargrupper vid Lunds universitet,Molecular Vascular Physiology,Lund University Research Groups,Thai Binh University of Medicine and Pharmacy(Swepub:lu)med-thr (author)
Binh, Bui ThiThai Binh University of Medicine and Pharmacy (author)
Phuong, Tran ThiChonnam National University (author)
Long, Phung ThanhNational Institute of Medicinal Materials, Vietnam (author)
Thu, Nguyen BichNational Institute of Medicinal Materials, Vietnam (author)
Que, Do Thi NguyetHanoi University of Pharmacy (author)
Khoi, Nguyen MinhNational Institute of Medicinal Materials, Vietnam (author)
Dung, Le VietNational Institute of Medicinal Materials, Vietnam (author)
Ngoc, Tran MinhNational Institute of Medicinal Materials, Vietnam (author)
National Institute of Medicinal Materials, VietnamMolekylär kärlfysiologi (creator_code:org_t)

By the author/editor: Dao, Trong Tuan; Ha, Do Thi; Hien Tran, Thi; Binh, Bui Thi; Phuong, Tran Thi; Long, Phung Than ...; show more...; Thu, Nguyen Bich; Que, Do Thi Nguy ...; Khoi, Nguyen Min ...; Dung, Le Viet; Ngoc, Tran Minh; show less...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Pharmacology and ...

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