Sökning: onr:"swepub:oai:lup.lub.lu.se:77bad997-cac5-42ea-af37-1c0d96b8ebad" >
IGF-I/insulin hybri...
IGF-I/insulin hybrid receptors in human endothelial cells
-
- Dekker Nitert, Marloes (författare)
- Linköpings universitet,Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine,Cellbiologi,Hälsouniversitetet
-
- Chisalitaa, Simona I., 1972- (författare)
- Linköpings universitet,Cellbiologi,Hälsouniversitetet
-
- Olsson, Karolina (författare)
- Linköpings universitet,Cellbiologi,Hälsouniversitetet
-
visa fler...
-
- Bornfeldt, Karin E (författare)
- Department of Pathology, University of Washington School of Medicine, Seattle, USA
-
- Arnqvist, Hans J. (författare)
- Linköpings universitet,Cellbiologi,Hälsouniversitetet
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2005
- 2005
- Engelska.
-
Ingår i: Molecular and Cellular Endocrinology. - : Elsevier BV. - 1872-8057 .- 0303-7207. ; 229:1-2, s. 31-37
- Relaterad länk:
-
http://dx.doi.org/10...
-
visa fler...
-
http://urn.kb.se/res...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- Vascular complications are common in diabetes. IGF-I receptors (IGF-IR) and insulin receptors (IR) in endothelial cells might respond to altered levels of IGF-I and insulin, resulting in altered endothelial function in diabetes. We therefore studied IGF-IR and IR gene expression, ligand binding, receptor protein, and phosphorylation in human umbilical vein endothelial cells (HUVEC). IGF-IR mRNA was more abundant than IR mRNA in freshly isolated HUVEC (IGF-IR/IR ratio 7.1 +/- 1.5) and in cultured HUVEC (ratio 3.5 +/- 0.51). Accordingly, specific binding of (125)I-IGF-I (0.64 +/- 0.25%) was higher than that of (125)I-insulin (0.25 +/- 0.09%). Protein was detected for both receptors and IGF-I/insulin hybrid receptors. IGF-IR phosphorylation was stimulated by 10(-10) to 10(-8) M IGF-I. IR were activated by 10(-9) to 10(-8) M insulin and IGF-I. We conclude that HUVEC express more IGF-IR than IR, and also express hybrid receptors. Both IGF-I and insulin phosphorylate their own receptors but only IGF-I seems to phosphorylate hybrid receptors.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Nyckelord
- Insulin/IGF-I hybrid receptor
- Endothelial cells
- Human
- Insulin receptor
- IGF-I receptor
- IGF-I receptor; Insulin receptor; Insulin/IGF-I hybrid receptor; Endothelial cells; Human
- MEDICINE
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas