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Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase
- Walfridsson, M (author)
- Bao, X (author)
-
- Anderlund, M (author)
- Lund University,Lunds universitet,Teknisk mikrobiologi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Applied Microbiology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- (creator_code:org_t)
- 1996
- 1996
- English 4 s.
- In: Applied and Environmental Microbiology. - 0099-2240. ; 62:12, s. 51-4648
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. The recombinant xylose isomerase showed the highest activity at 85 degrees C with a specific activity of 1.0 U mg-1. A new functional metabolic pathway in S. cerevisiae with ethanol formation during oxygen-limited xylose fermentation was demonstrated. Xylitol and acetic acid were also formed during the fermentation.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- Aldose-Ketose Isomerases
- Base Sequence
- Carbohydrate Epimerases
- Ethanol
- Fermentation
- Genes, Bacterial
- Molecular Sequence Data
- Phosphoglycerate Kinase
- Recombinant Proteins
- Saccharomyces cerevisiae
- Thermus thermophilus
- Xylose
Publication and Content Type
- art (subject category)
- ref (subject category)
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- Walfridsson, M
- Bao, X
- Anderlund, M
- Lilius, G
- Bülow, L
- Hahn-Hägerdal, B
- About the subject
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- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- and Microbiology
- Articles in the publication
- Applied and Envi ...
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- Lund University
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