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Importance of the N...
Importance of the N-terminal sequence in porcine pancreatic colipase
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- Erlanson-Albertsson, C (author)
- Lund University,Lunds universitet,Aptitkontroll,Forskargrupper vid Lunds universitet,Appetite Regulation,Lund University Research Groups
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Larsson, A (author)
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(creator_code:org_t)
- Elsevier BV, 1981
- 1981
- English.
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In: Biochimica et Biophysica Acta. - : Elsevier BV. - 0006-3002. ; 665:2, s. 5-250
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Abstract
Subject headings
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- Colipase exists in pancreatic juice in a pro-form which is activated by limited trypsin hydrolysis. During this activation, the N-terminal pentapeptide 1Val-Pro-Asp-Pro-5Arg is cleaved. The new N-terminal sequence formed, 6Gly-Ile-Ile-Ile-10Asn, contains three isoleucine residues. The importance of these for stimulating lipase activity has been investigated by successive Edman degradation of epsilon-acetimidolysine residues followed by limited trypsin hydrolysis. The epsilon-amidinated colipase obtained was fully active both with a phospholipid-covered triacylglycerol (Intralipid) and tributyrin as substrate. After removal of the three isoleucine residues, the activity of colipase was lost with Intralipid but not with tributyrin as substrate. The shortened colipases regained their Intralipid activity upon addition of long-chain fatty acids. The binding of colipase to lipase was not affected by removal of the isoleucine residues.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Keyword
- Amino Acid Sequence
- Animals
- Colipases/pharmacology
- Lipase/metabolism
- Pancreas/analysis
- Peptide Fragments/analysis
- Protein Binding
- Proteins/pharmacology
- Swine
- Trypsin
Publication and Content Type
- art (subject category)
- ref (subject category)
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