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Generation of human...
Generation of human-induced pluripotent-stem-cell-derived cortical neurons for high-throughput imaging of neurite morphology and neuron maturation
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- Wali, Gautam (author)
- Kolling Institute of Medical Research,University of New South Wales
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- Li, Yan (author)
- University of New South Wales,Kolling Institute of Medical Research
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- Abu-Bonsrah, Dad (author)
- University of Melbourne
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- Kirik, Deniz (author)
- Lund University,Lunds universitet,Brain Repair and Imaging in Neural Systems (BRAINS),Forskargrupper vid Lunds universitet,Lund University Research Groups,University of Sydney
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- Parish, Clare L. (author)
- University of Melbourne
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- Sue, Carolyn M. (author)
- University of Sydney,Aligning Science Across Parkinson's (ASAP) Collaborative Research Network,University of New South Wales,Kolling Institute of Medical Research
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(creator_code:org_t)
- 2023
- 2023
- English.
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In: STAR Protocols. - 2666-1667. ; 4:2
- Related links:
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Subject headings
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- High-throughput imaging allows in vitro assessment of neuron morphology for screening populations under developmental, homeostatic, and/or disease conditions. Here, we present a protocol to differentiate cryopreserved human cortical neuronal progenitors into mature cortical neurons for high-throughput imaging analysis. We describe the use of a notch signaling inhibitor to generate homogeneous neuronal populations at densities amenable to individual neurite identification. We detail neurite morphology assessment via measuring multiple parameters including neurite length, branches, roots, segments and extremities, and neuron maturation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- Cell Biology
- Cell culture
- Cell Differentiation
- Microscopy
- Neuroscience
- Stem Cells
Publication and Content Type
- art (subject category)
- ref (subject category)
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