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Human alveolar hydr...
Human alveolar hydrogels promote morphological and transcriptional differentiation in iPSC-derived alveolar type 2 epithelial cells
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- Hoffman, Evan T. (author)
- University of Vermont
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- Uriarte, Juan J. (author)
- University of Kentucky
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- Uhl, Franziska E. (author)
- Lund University,Lunds universitet,Vaskulär biologi,Forskargrupper vid Lunds universitet,Vascular Biology,Lund University Research Groups
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- Eckstrom, Korin (author)
- University of Vermont
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- Tanneberger, Alicia E. (author)
- University of Vermont
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- Becker, Chloe (author)
- University of Vermont
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- Moulin, Chloe (author)
- University of Vermont
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- Asarian, Loredana (author)
- University of Vermont
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- Ikonomou, Laertis (author)
- The State University of New York
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- Kotton, Darrell N. (author)
- Boston Medical Center
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- Weiss, Daniel J. (author)
- University of Vermont
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(creator_code:org_t)
- 2023
- 2023
- English.
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In: Scientific Reports. - 2045-2322. ; 13:1
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http://dx.doi.org/10... (free)
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Abstract
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- Alveolar type 2 epithelial cells (AT2s) derived from human induced pluripotent stem cells (iAT2s) have rapidly contributed to our understanding of AT2 function and disease. However, while iAT2s are primarily cultured in three-dimensional (3D) Matrigel, a matrix derived from cancerous mouse tissue, it is unclear how a physiologically relevant matrix will impact iAT2s phenotype. As extracellular matrix (ECM) is recognized as a vital component in directing cellular function and differentiation, we sought to derive hydrogels from decellularized human lung alveolar-enriched ECM (aECM) to provide an ex vivo model to characterize the role of physiologically relevant ECM on iAT2 phenotype. We demonstrate aECM hydrogels retain critical in situ ECM components, including structural and basement membrane proteins. While aECM hydrogels facilitate iAT2 proliferation and alveolosphere formation, a subset of iAT2s rapidly change morphology to thin and elongated ring-like cells. This morphological change correlates with upregulation of recently described iAT2-derived transitional cell state genetic markers. As such, we demonstrate a potentially underappreciated role of physiologically relevant aECM in iAT2 differentiation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
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- art (subject category)
- ref (subject category)
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- By the author/editor
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Hoffman, Evan T.
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Uriarte, Juan J.
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Uhl, Franziska E ...
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Eckstrom, Korin
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Tanneberger, Ali ...
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Becker, Chloe
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show more...
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Moulin, Chloe
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Asarian, Loredan ...
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Ikonomou, Laerti ...
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Kotton, Darrell ...
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Weiss, Daniel J.
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Scientific Repor ...
- By the university
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Lund University