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Crystal structure o...
Crystal structure of tubulin folding cofactor A from Arabidopsis thaliana and its beta-tubulin binding characterization
- Article/chapterEnglish2010
Publisher, publication year, extent ...
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2010-07-16
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Wiley,2010
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7 s.
Numbers
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LIBRIS-ID:oai:lup.lub.lu.se:88c0406d-5a12-4137-a2af-387abdfc8bfa
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https://lup.lub.lu.se/record/88c0406d-5a12-4137-a2af-387abdfc8bfaURI
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https://doi.org/10.1016/j.febslet.2010.07.017DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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Microtubules are composed of polymerized alpha/beta-tubulin heterodimers. Biogenesis of assembly-competent tubulin dimers is a complex multistep process that requires sequential actions of distinct molecular chaperones and cofactors. Tubulin folding cofactor A (TFCA), which captures beta-tubulin during the folding pathway, has been identified in many organisms. Here, we report the crystal structure of Arabidopsis thaliana TFC A (KIESEL, KIS), which forms a monomeric three-helix bundle. The functional binding analysis demonstrated that KIS interacts with beta-tubulin in plant. Furthermore, mutagenesis studies indicated that the alpha-helical regions of KIS participate in beta-tubulin binding. Unlike the budding yeast TFC A, the two loop regions of KIS are not required for this interaction suggesting a distinct binding mechanism of TFC A to beta-tubulin in plants.
Subject headings and genre
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Amino Acid Sequence
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Arabidopsis
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Arabidopsis Proteins
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Crystallography, X-Ray
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Genes, Plant
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Genetic Complementation Test
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Microtubule-Associated Proteins
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Models, Molecular
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Molecular Chaperones
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Plants, Genetically Modified
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Protein Binding
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Protein Folding
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Protein Interaction Domains and Motifs
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Protein Structure, Secondary
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Recombinant Proteins
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Sequence Homology, Amino Acid
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Tubulin
Added entries (persons, corporate bodies, meetings, titles ...)
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Nan, JieLund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory,Peking University(Swepub:lu)maxl-jna
(author)
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Mi, Wei
(author)
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Li, Lan-Fen
(author)
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Wei, Chun-Hong
(author)
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Su, Xiao-DongLund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)mbfys-xs
(author)
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Li, Yi
(author)
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Lunds universitetMAX IV-laboratoriet
(creator_code:org_t)
Related titles
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In:FEBS Letters: Wiley584:16, s. 9-35331873-34680014-5793
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