Thyroid-beta2 and the retinoid RAR-alpha, RXR-gamma and ROR-beta2 receptor mRNAs; expression profiles in mouse retina, retinal explants and neocortex.

• In neonatal retinal explants cultured long-term green cones are missing. Recently it was reported that thyroid hormone beta2 receptors (TR-beta2) are essential for these green cones to differentiate. Therefore transcript level of these receptors was investigated in our mouse retinal explants. However, thyroid receptors function as heterodimers with retinoid receptors (RR); so the fate of selected RRs was similarly analyzed using semi-quantitative RT-PCR. Loss of TR-beta2 and RR (RXR-gamma and ROR-beta2) mRNAs was observed after culturing the neonatal retina for 12 days. This indicates that these proteins are involved in determination of green cone identity. In addition, levels of the selected RR transcripts are differentially affected by short- or long-term culture. In the latter case an attached retinal pigment epithelium seems to play a protective role. Furthermore, divergent diurnal peaks of RR mRNAs are present in young as well as aged mouse retina and neocortex. This data might be relevant in the context of human ageing disorders.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Neurosciences (hsv//eng)

Keyword

Mice

Inbred C3H

Neocortex : cytology

Neocortex : growth & development

Neocortex : metabolism

Organ Culture : methods

RNA

Messenger : metabolism

Receptors

Cell Surface : genetics

Retinoic Acid : genetics

Thyroid Hormone : genetics

Retina : cytology

Retina : metabolism

Retina : growth & development

Support

Non-U.S. Gov't

Transcription Factors : genetics

Transcription

Genetic : physiology

Up-Regulation : genetics

Male

Gene Expression Regulation : physiology

Female

Down-Regulation : genetics

Dark Adaptation : genetics

Circadian Rhythm : genetics

Cell Differentiation : genetics

Animal

Aging : metabolism

Publication and Content Type

art (subject category)

ref (subject category)