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Rapid and effective...
Rapid and effective enrichment of mononuclear cells from blood using acoustophoresis
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- Urbansky, Anke (author)
- Lund University,Lunds universitet,Acoustofluidics group,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Ohlsson, Pelle (author)
- Lund University,Lunds universitet,Acoustofluidics group,Forskargrupper vid Lunds universitet,Lund University Research Groups,AcouSort AB
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- Lenshof, Andreas (author)
- Lund University,Lunds universitet,Acoustofluidics group,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Garofalo, Fabio (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Scheding, Stefan (author)
- Lund University,Lunds universitet,Avdelningen för molekylär hematologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Molecular Hematology (DMH),Department of Laboratory Medicine,Faculty of Medicine,Skåne University Hospital
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- Laurell, Thomas (author)
- Lund University,Lunds universitet,Acoustofluidics group,Forskargrupper vid Lunds universitet,Lund University Research Groups,Dongguk University, Seoul
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(creator_code:org_t)
- 2017-12-07
- 2017
- English.
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In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7:1
- Related links:
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Abstract
Subject headings
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- Effective separation methods for fractionating blood components are needed for numerous diagnostic and research applications. This paper presents the use of acoustophoresis, an ultrasound based microfluidic separation technology, for label-free, gentle and continuous separation of mononuclear cells (MNCs) from diluted whole blood. Red blood cells (RBCs) and MNCs behave similar in an acoustic standing wave field, compromising acoustic separation of MNC from RBC in standard buffer systems. However, by optimizing the buffer conditions and thereby changing the acoustophoretic mobility of the cells, we were able to enrich MNCs relative to RBCs by a factor of 2,800 with MNC recoveries up to 88%. The acoustophoretic microchip can perform cell separation at a processing rate of more than 1 × 105 cells/s, corresponding to 5 μl/min undiluted whole blood equivalent. Thus, acoustophoresis can be easily integrated with further down-stream applications such as flow cytometry, making it a superior alternative to existing MNC isolation techniques.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk laboratorie- och mätteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Laboratory and Measurements Technologies (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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