Search: onr:"swepub:oai:lup.lub.lu.se:91097b12-2bfa-444c-81e2-6813b2a02270" >
Beneficial effects ...
Beneficial effects of alpha-1 antitrypsin therapy in a mouse model of colitis-associated colon cancer
-
- Al-Omari, Mariam (author)
- Yarmouk University
-
- Al-Omari, Tareq (author)
- Yarmouk University
-
- Batainah, Nesreen (author)
- Yarmouk University
-
show more...
-
- Al-Qauod, Khaled (author)
- Yarmouk University
-
- Olejnicka, Beata (author)
- Hannover Medical School
-
- Janciauskiene, Sabina (author)
- Lund University,Lunds universitet,Internmedicin - epidemiologi,Forskargrupper vid Lunds universitet,Internal Medicine - Epidemiology,Lund University Research Groups,Hannover Medical School
-
show less...
-
(creator_code:org_t)
- 2023
- 2023
- English.
-
In: BMC Cancer. - 1471-2407. ; 23:1
- Related links:
-
http://dx.doi.org/10... (free)
-
show more...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Background: It is widely accepted that chronic inflammatory bowel diseases significantly higher a risk for colorectal cancer development. Among different types of treatments for patients with colon cancer, novel protein-based therapeutic strategies are considered. AIM: To explore the effect of human plasma alpha-1 antitrypsin (AAT) protein in the chemically induced mouse model of colorectal cancer. Methods: BALB/c mice with azoxymethane/dextran sodium sulfate (AOM/DSS)-induced colitis-associated colorectal cancer (CAC), we intraperitoneally treated with commercial preparation of human plasma AAT (4 mg per mouse). Effects of this therapy were evaluated histologically, and by immunohistochemical and gene expression assays. Results: When compared with non-treated controls, AOM/DSS mice receiving AAT therapy exhibited significantly longer colons, and less anal bleeding. Concurrently, AAT-treated mice had significantly fewer polyps, and lower numbers of large colon tumors. Immunohistochemical examinations of colon tissues showed significantly lower neutrophil counts, more granzyme B-positive but fewer MMP9 (gelatinase B)-positive cancer cells and lower numbers of apoptotic cells in mice receiving AAT therapy. The expression levels of IL4 were significantly higher while TNFA was slightly reduced in tumor tissues of AOM/DSS mice treated with AAT than in AOM/DSS mice. Conclusion: Human AAT is an acute phase protein with a broad-protease inhibitory and immunomodulatory activities used as a therapeutic for emphysema patients with inherited AAT deficiency. Our results are consistent with previous findings and support an idea that AAT alone and/or in combination with available anti-cancer therapies may represent a new personalized approach for patients with colitis-induced colon cancer.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- Alpha1-antitrypsin
- Caspase-3
- Colorectal cancer
- Cytokines
- Granzyme-B
- Inflammation
- Matrix metalloproteinases
- Mice model
- Neutrophils
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
-
BMC Cancer
(Search for host publication in LIBRIS)
To the university's database