The Mitochondrial DNA (mtDNA)-Associated Protein SWIB5 Influences mtDNA Architecture and Homologous Recombination

In addition to the nucleus, mitochondria and chloroplasts in plant cells also contain genomes. Efficient DNA repair pathways are crucial in these organelles to fix damage resulting from endogenous and exogenous factors. Plant organellar genomes are complex compared to their animal counterparts and although several plant-specific mediators of organelle DNA repair have been reported, many regulators remain to be identified. Here, we show that a mitochondrial SWI/SNF (nucleosome remodeling) complex B protein, SWIB5, is capable of associating with mitochondrial DNA (mtDNA) in Arabidopsis thaliana. Gain- and loss-of-function mutants provided evidence for a role of SWIB5 in influencing mtDNA architecture and homologous recombination at specific intermediate-sized repeats both under normal and genotoxic conditions. SWIB5 interacts with other mitochondrial SWIB proteins. Gene expression and mutant phenotypic analysis of SWIB5 and SWIB family members suggests a link between organellar genome maintenance and cell proliferation. Taken together, our work presents a protein family that influences mtDNA architecture and homologous recombination in plants and suggests a link between organelle functioning and plant development.

Van Aken, OlivierLund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Molecular Cell Biology,Department of Biology,Faculty of Science,University of Western Australia, Crawley(Swepub:lu)ol4538va (author)
Van Leene, JelleGhent University (author)
Jégu, TeddyUniversity of Paris-Saclay (author)
De Rycke, Riet MariaGhent University (author)
De Bruyne, MichielGhent University (author)
Vercruysse, JasmienGhent University (author)
Nolf, JonahGhent University (author)
Van Daele, TwiggyGhent University (author)
De Milde, LiesbethGhent University (author)
Vermeersch, MattiasGhent University (author)
Colas des Francs-Small, CatherineUniversity of Western Australia, Crawley (author)
De Jaeger, GeertGhent University (author)
Benhamed, MoussaUniversity of Paris-Saclay (author)
Millar, A. HarveyUniversity of Western Australia, Crawley (author)
Inzé, DirkGhent University (author)
Gonzalez, NathalieFlanders Interuniversity Institute for Biotechnology,Ghent University (author)
Ghent UniversityMolekylär cellbiologi (creator_code:org_t)

In:Plant Cell: Oxford University Press (OUP)29:5, s. 1137-11561040-46511532-298X

By the author/editor: Blomme, Jonas; Van Aken, Olivie ...; Van Leene, Jelle; Jégu, Teddy; De Rycke, Riet M ...; De Bruyne, Michi ...; show more...; Vercruysse, Jasm ...; Nolf, Jonah; Van Daele, Twigg ...; De Milde, Liesbe ...; Vermeersch, Matt ...; Colas des Francs ...; De Jaeger, Geert; Benhamed, Moussa; Millar, A. Harve ...; Inzé, Dirk; Gonzalez, Nathal ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

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