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MicroRNAs in Type 2...
MicroRNAs in Type 2 Diabetes : Focus on MicroRNA Profiling in Islets of Langerhans
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- Cowan, Elaine (author)
- Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Diabetes - öcellsexocytos,Forskargrupper vid Lunds universitet,Department of Clinical Sciences, Malmö,Faculty of Medicine,Diabetes - Islet Cell Exocytosis,Lund University Research Groups,Skåne University Hospital
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- Karagiannopoulos, Alexandros (author)
- Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Diabetes - öcellsexocytos,Forskargrupper vid Lunds universitet,Department of Clinical Sciences, Malmö,Faculty of Medicine,Diabetes - Islet Cell Exocytosis,Lund University Research Groups,Skåne University Hospital
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- Eliasson, Lena (author)
- Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Diabetes - öcellsexocytos,Forskargrupper vid Lunds universitet,Department of Clinical Sciences, Malmö,Faculty of Medicine,Diabetes - Islet Cell Exocytosis,Lund University Research Groups,Skåne University Hospital
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Moore, Anna (editor)
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Wang, Ping (editor)
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(creator_code:org_t)
- 2022-12-13
- 2022
- English 30 s.
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In: Type-1 Diabetes : Methods and Protocols - Methods and Protocols. - New York, NY : Springer US. - 1940-6029. - 9781071628072 - 9781071628065 ; :2592, s. 113-142
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Abstract
Subject headings
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- Differential expression of microRNAs (miRNAs) is observed in many diseases including type 2 diabetes (T2D). Insulin secretion from pancreatic beta cells is central for the regulation of blood glucose levels and failure to release enough insulin results in hyperglycemia and T2D. The importance in T2D pathogenesis of single miRNAs in beta cells has been described; however, to get the full picture, high-throughput miRNA sequencing is necessary. Here we describe a method using small RNA sequencing, from sample preparation to expression analysis using bioinformatic tools. In the end, a tutorial on differential expression analysis is presented in R using publicly available data.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Keyword
- Islets of Langerhans
- Beta-cell
- Insulin secretion
- MicroRNA
- Diabetes
- Cardiometabolic disease
- RNA sequencing
- Short noncoding RNA
- Expression analyses
Publication and Content Type
- kap (subject category)
- ref (subject category)
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