MicroRNAs in Type 2 Diabetes : Focus on MicroRNA Profiling in Islets of Langerhans

Cowan, ElaineLund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Diabetes - öcellsexocytos,Forskargrupper vid Lunds universitet,Department of Clinical Sciences, Malmö,Faculty of Medicine,Diabetes - Islet Cell Exocytosis,Lund University Research Groups,Skåne University Hospital (author)

Differential expression of microRNAs (miRNAs) is observed in many diseases including type 2 diabetes (T2D). Insulin secretion from pancreatic beta cells is central for the regulation of blood glucose levels and failure to release enough insulin results in hyperglycemia and T2D. The importance in T2D pathogenesis of single miRNAs in beta cells has been described; however, to get the full picture, high-throughput miRNA sequencing is necessary. Here we describe a method using small RNA sequencing, from sample preparation to expression analysis using bioinformatic tools. In the end, a tutorial on differential expression analysis is presented in R using publicly available data.

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Endokrinologi och diabetes hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Endocrinology and Diabetes hsv//eng
Islets of Langerhans
Beta-cell
Insulin secretion
MicroRNA
Diabetes
Cardiometabolic disease
RNA sequencing
Short noncoding RNA
Expression analyses

Karagiannopoulos, AlexandrosLund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Diabetes - öcellsexocytos,Forskargrupper vid Lunds universitet,Department of Clinical Sciences, Malmö,Faculty of Medicine,Diabetes - Islet Cell Exocytosis,Lund University Research Groups,Skåne University Hospital(Swepub:lu)al6183ka (author)
Eliasson, LenaLund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Diabetes - öcellsexocytos,Forskargrupper vid Lunds universitet,Department of Clinical Sciences, Malmö,Faculty of Medicine,Diabetes - Islet Cell Exocytosis,Lund University Research Groups,Skåne University Hospital(Swepub:lu)mphy-lel (author)
Moore, Anna (editor)
Wang, Ping (editor)
Institutionen för kliniska vetenskaper, MalmöMedicinska fakulteten (creator_code:org_t)

In:Type-1 Diabetes : Methods and Protocols - Methods and ProtocolsNew York, NY : Springer US:2592, s. 113-1421940-602997810716280659781071628072

By the author/editor: Cowan, Elaine; Karagiannopoulos ...; Eliasson, Lena; Moore, Anna; Wang, Ping

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Endocrinology an ...

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