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- Li, LanfenPeking University (author)
Structural genomics studies of human caries pathogen Streptococcus mutans
- Article/chapterEnglish2014
Publisher, publication year, extent ...
- 2014-01-29
- Springer Science and Business Media LLC,2014
- 9 s.
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- LIBRIS-ID:oai:lup.lub.lu.se:981bedcd-0602-4dcb-876f-de967a8de046
- https://lup.lub.lu.se/record/981bedcd-0602-4dcb-876f-de967a8de046URI
- https://doi.org/10.1007/s10969-014-9172-3DOI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
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Notes
- Gram-positive bacterium Streptococcus mutans is the primary causative agent of human dental caries. To better understand this pathogen at the atomic structure level and to establish potential drug and vaccine targets, we have carried out structural genomics research since 2005. To achieve the goal, we have developed various in-house automation systems including novel high-throughput crystallization equipment and methods, based on which a large-scale, high-efficiency and low-cost platform has been establish in our laboratory. From a total of 1,963 annotated open reading frames, 1,391 non-membrane targets were selected prioritized by protein sequence similarities to unknown structures, and clustered by restriction sites to allow for cost-effective high-throughput conventional cloning. Selected proteins were over-expressed in different strains of Escherichia coli. Clones expressed soluble proteins were selected, expanded, and expressed proteins were purified and subjected to crystallization trials. Finally, protein crystals were subjected to X-ray analysis and structures were determined by crystallographic methods. Using the previously established procedures, we have so far obtained more than 200 kinds of protein crystals and 100 kinds of crystal structures involved in different biological pathways. In this paper we demonstrate and review a possibility of performing structural genomics studies at moderate laboratory scale. Furthermore, the techniques and methods developed in our study can be widely applied to conventional structural biology research practice.
Subject headings and genre
- Bacterial Proteins
- Cloning, Molecular
- Computational Biology
- Crystallization
- Crystallography, X-Ray
- Dental Caries
- Genome, Bacterial
- Genomics
- Humans
- Image Interpretation, Computer-Assisted
- Proteomics
- Streptococcus mutans
Added entries (persons, corporate bodies, meetings, titles ...)
- Nan, JieLund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory,Peking University(Swepub:lu)maxl-jna (author)
- Li, DanPeking University (author)
- Brostromer, ErikPeking University (author)
- Wang, ZixiPeking University (author)
- Liu, CongPeking University (author)
- Hou, QiaomingPeking University (author)
- Fan, XuexinPeking University (author)
- Ye, ZhaoyangPeking University (author)
- Su, Xiao-DongLund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Peking University(Swepub:lu)mbfys-xs (author)
- Peking UniversityMAX IV-laboratoriet (creator_code:org_t)
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- In:Journal of Structural and Functional Genomics: Springer Science and Business Media LLC15:3, s. 9-911345-711X1570-0267
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