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  • Brolen, Gabriella (author)

Hepatocyte-like cells derived from human embryonic stem cells specifically via definitive endoderm and a progenitor stage

  • Article/chapterEnglish2010

Publisher, publication year, extent ...

  • Elsevier BV,2010

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:9a579750-8d30-43e4-b6d6-f8267e2ee4a9
  • https://lup.lub.lu.se/record/1568139URI
  • https://doi.org/10.1016/j.jbiotec.2009.11.007DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:120086259URI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • Human embryonic stem cells offer a potential unlimited supply for functional hepatocytes, since they can differentiate into hepatocyte-like cells displaying a characteristic hepatic morphology and expressing various hepatic markers. These cells could be used in various applications such as studies of drug metabolism and hepatotoxicity, which however, would require a significant expression of drug metabolizing enzymes. To derive these cells we use a stepwise differentiation protocol where growth- and maturation factors are added. The first phase involves the formation of definitive endoderm. Next, these cells are treated with factors known to promote the induction and proliferation towards hepatic progenitor cell types. In the last phase the cells are terminally differentiated and maturated into functional hepatocyte-like cells. The cultures were characterized by analysis of endodermal or hepatic markers and compared to cultures derived without induction via definitive endoderm. Hepatic functions such as urea secretion, glycogen storage, indocyanine green uptake and secretion, and cytochrome P450-expression and activity were evaluated. The DE-Hep showed a hepatocyte morphology with sub-organized cells and exhibited many liver-functions including transporter activity and capacity to metabolize drugs specific for important cytochrome P450 sub-families. This represents an importantstep in differentiation of hESC into functional hepatocytes. (C) 2009 Elsevier B.V. All rights reserved.

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  • Sivertsson, LouiseKarolinska Institutet (author)
  • Bjorquist, Petter (author)
  • Eriksson, Gustav (author)
  • Ek, Monica (author)
  • Semb, HenrikLund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)endo-hse (author)
  • Johansson, IngerKarolinska Institutet (author)
  • Andersson, Tommy B. (author)
  • Ingelman-Sundberg, MagnusKarolinska Institutet (author)
  • Heins, Nico (author)
  • Karolinska InstitutetStamcellscentrum (SCC) (creator_code:org_t)

Related titles

  • In:Journal of Biotechnology: Elsevier BV145:3, s. 284-2941873-48630168-1656

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