Sökning: onr:"swepub:oai:lup.lub.lu.se:9caf6db1-d29f-402b-85b8-4c059ad82369" >
Characterization of...
-
Becker, CharlotteLund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups
(författare)
Characterization of epitope structure for 53 monoclonal antibodies against prostate-specific antigen
- Artikel/kapitelEngelska1999
Förlag, utgivningsår, omfång ...
-
Springer Science and Business Media LLC,1999
Nummerbeteckningar
-
LIBRIS-ID:oai:lup.lub.lu.se:9caf6db1-d29f-402b-85b8-4c059ad82369
-
https://lup.lub.lu.se/record/1115150URI
-
https://doi.org/10.1159/000056524DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:art swepub-publicationtype
-
Ämneskategori:ref swepub-contenttype
Anmärkningar
-
Prostate-specific antigen (PSA) is the most widely used marker of prostate cancer. Assays for PSA are based on anti-PSA antibodies, and the characterization and selection of these antibodies is important for determining their optimum performance. In our study, we characterized the reactivity of 53 antibodies, submitted to the ISOBM TD-3 PSA Workshop, using free PSA, PSA complexed to alpha1-antichymotrypsin (ACT) and purified ACT. Immunoblotting was performed after native agarose gel or reducing sodium dodecyl polyacrylamide gel electrophoresis. Immunoblotting after agarose gel electrophoresis revealed 10 antibodies that recognized only the free form of PSA, and 43 antibodies that detected both free PSA and PSA-ACT. Immunoblotting of reducing sodium dodecyl-polyacrylamide gels showed the linear or conformation-dependent nature of the epitopes. Two antibodies specific for free PSA and 18 antibodies that recognized both free PSA and PSA-ACT complex recognized linear epitopes. Moreover, 7 antibodies also detected fragmented forms of PSA.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Wigheden, IngridLund University,Lunds universitet,Celiaki och diabetes,Forskargrupper vid Lunds universitet,Celiac Disease and Diabetes Unit,Lund University Research Groups(Swepub:lu)klke-iwi
(författare)
-
Lilja, HansLund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups(Swepub:lu)klke-hli
(författare)
-
Klinisk kemi, MalmöForskargrupper vid Lunds universitet
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Tumor Biology: Springer Science and Business Media LLC20:Suppl. 1, s. 13-171423-03801010-4283
Internetlänk
Hitta via bibliotek
Till lärosätets databas