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Poly(hydroxyethyl m...
Poly(hydroxyethyl methacrylate)-based composite cryogel with embedded macroporous cellulose beads for the separation of human serum immunoglobulin and albumin
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Ye, Jialei (author)
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Yun, Junxian (author)
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Lin, Dong-Qiang (author)
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Xu, Linhong (author)
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- Kirsebom, Harald (author)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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Shen, Shaochuan (author)
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Yang, Gensheng (author)
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Yao, Kejian (author)
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Guan, Yi-Xin (author)
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Yao, Shan-Jing (author)
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(creator_code:org_t)
- 2013-11-18
- 2013
- English.
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In: Journal of Separation Science. - : Wiley. - 1615-9314 .- 1615-9306. ; 36:24, s. 3813-3820
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http://dx.doi.org/10...
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Abstract
Subject headings
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- A novel super-macroporous monolithic composite cryogel was prepared by embedding macroporous cellulose beads into poly(hydroxyethyl methacrylate) cryogel. The cellulose beads were fabricated by using a microchannel liquid-flow focusing and cryopolymerization method, while the composite cryogel was prepared by cryogenic radical polymerization of the hydroxyethyl methacrylate monomer with poly(ethylene glycol) diacrylate as cross-linker together with the cellulose beads. After graft polymerization with (vinylbenzyl)trimethylammonium chloride, the composite cryogel was applied to separate immunoglobulin-G and albumin from human serum. Immunoglobulin-G with a mean purity of 83.2% and albumin with a purity of 98% were obtained, indicating the composite cryogel as a promising chromatographic medium in bioseparation for the isolation of important bioactive proteins like immunoglobulins and albumins.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Keyword
- Bioseparation
- Cellulose beads
- Cryogels
- Immunoglobulin-G
- Ion-exchange
- adsorption
Publication and Content Type
- art (subject category)
- ref (subject category)
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To the university's database
- By the author/editor
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Ye, Jialei
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Yun, Junxian
-
Lin, Dong-Qiang
-
Xu, Linhong
-
Kirsebom, Harald
-
Shen, Shaochuan
-
show more...
-
Yang, Gensheng
-
Yao, Kejian
-
Guan, Yi-Xin
-
Yao, Shan-Jing
-
show less...
- About the subject
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
- Articles in the publication
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Journal of Separ ...
- By the university
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Lund University