Search: onr:"swepub:oai:lup.lub.lu.se:a01fba00-e273-48b3-9682-48dab9db8ec6" >
Conformational anal...
Conformational analysis of HAMLET, the folding variant of human alpha-lactalbumin associated with apoptosis
- Article/chapterEnglish2004
Publisher, publication year, extent ...
Numbers
-
LIBRIS-ID:oai:lup.lub.lu.se:a01fba00-e273-48b3-9682-48dab9db8ec6
-
https://lup.lub.lu.se/record/280093URI
-
https://doi.org/10.1110/ps.03474704DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:art swepub-publicationtype
-
Subject category:ref swepub-contenttype
Notes
-
A combination of hydrogen/deuterium (H/D) exchange and limited proteolysis experiments coupled to mass spectrometry analysis was used to depict the conformation in solution of HAMLET, the folding variant of human alpha-lactalbumin, complexed to oleic acid, that induces apoptosis in tumor and immature cells. Although near- and far-UV CD and fluorescence spectroscopy were not able to discriminate between HAMLET and apo-alpha-lactalbumin, H/D exchange experiments clearly showed that they correspond to two distinct conformational states, with HAMLET incorporating a greater number of deuterium atoms than the apo and holo forms. Complementary proteolysis experiments revealed that HAMLET and apo are both accessible to proteases in the P-domain but showed substantial differences in accessibility to proteases at specific sites. The overall results indicated that the conformational changes associated with the release of Ca2+ are not sufficient to induce the HAMLET conformation. Metal depletion might represent the first event to produce a partial unfolding in the beta-domain of a-lactalbumin, but some more unfolding is needed to generate the active conformation HAMLET, very likely allowing the protein to bind the C18:1 fatty acid moiety. On the basis of these data, a putative binding site of the oleic acid, which stabilizes the HAMLET conformation, is proposed.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Birolo, L
(author)
-
Infusini, G
(author)
-
DAL Piaz, F
(author)
-
Svensson, M
(author)
-
Pucci, P
(author)
-
Svanborg, CatharinaLund University,Lunds universitet,Avdelningen för mikrobiologi, immunologi och glykobiologi - MIG,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Microbiology, Immunology and Glycobiology - MIG,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)mmb-csv
(author)
-
Marino, G
(author)
-
Avdelningen för mikrobiologi, immunologi och glykobiologi - MIGInstitutionen för laboratoriemedicin
(creator_code:org_t)
Related titles
-
In:Protein Science: Wiley13:5, s. 1322-13301469-896X0961-8368
Internet link
Find in a library
To the university's database