Search: onr:"swepub:oai:lup.lub.lu.se:a1b7f8f0-81a2-4cae-8e50-b8812825729d" >
Extension of nanoco...
Extension of nanoconfined DNA: Quantitative comparison between experiment and theory
-
- Iarko, V. (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)
-
- Werner, Erik (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)
-
- Nyberg, Lena, 1979 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
show more...
-
- Müller, Vilhelm, 1990 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Fritzsche, Joachim, 1977 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Ambjörnsson, Tobias (author)
- Lund University,Lunds universitet,Beräkningsbiologi och biologisk fysik - Genomgår omorganisation,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Computational Biology and Biological Physics - Undergoing reorganization,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science
-
- Beech, Jason (author)
- Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH
-
- Tegenfeldt, Jonas (author)
- Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH
-
- Mehlig, Kirsten, 1964 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för samhällsmedicin och folkhälsa, enheten för folkhälsoepidemiologi,Institute of Medicine, Department of Public Health and Community Medicine, Public Health Epidemiology Unit
-
- Westerlund, Fredrik, 1978 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Mehlig, Bernhard, 1964 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)
-
show less...
-
(creator_code:org_t)
- 2015
- 2015
- English.
-
In: Physical Review E (Statistical, Nonlinear, and Soft Matter Physics). - 1539-3755 .- 1550-2376. ; 92:6, s. Art. Nr. 062701-
- Related links:
-
http://dx.doi.org/10...
-
show more...
-
http://publications.... (primary) (free)
-
https://gup.ub.gu.se... (primary) (free)
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
https://research.cha...
-
https://gup.ub.gu.se...
-
show less...
Abstract
Subject headings
Close
- The extension of DNA confined to nanochannels has been studied intensively and in detail. However, quantitative comparisons between experiments and model calculations are difficult because most theoretical predictions involve undetermined prefactors, and because the model parameters (contour length, Kuhn length, effective width) are difficult to compute reliably, leading to substantial uncertainties. Here we use a recent asymptotically exact theory for the DNA extension in the "extended de Gennes regime" that allows us to compare experimental results with theory. For this purpose, we performed experiments measuring the mean DNA extension and its standard deviation while varying the channel geometry, dye intercalation ratio, and ionic strength of the buffer. The experimental results agree very well with theory at high ionic strengths, indicating that the model parameters are reliable. At low ionic strengths, the agreement is less good. We discuss possible reasons. In principle, our approach allows us to measure the Kuhn length and the effective width of a single DNA molecule and more generally of semiflexible polymers in solution.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Nanoteknik -- Nanoteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Nano-technology -- Nano-technology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database
- By the author/editor
-
Iarko, V.
-
Werner, Erik
-
Nyberg, Lena, 19 ...
-
Müller, Vilhelm, ...
-
Fritzsche, Joach ...
-
Ambjörnsson, Tob ...
-
show more...
-
Beech, Jason
-
Tegenfeldt, Jona ...
-
Mehlig, Kirsten, ...
-
Westerlund, Fred ...
-
Mehlig, Bernhard ...
-
show less...
- About the subject
-
- ENGINEERING AND TECHNOLOGY
-
ENGINEERING AND ...
-
and Nano technology
-
and Nano technology
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Biophysics
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Physical Science ...
-
and Other Physics To ...
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Physical Science ...
- Articles in the publication
-
Physical Review ...
-
Physical Review ...
- By the university
-
Lund University
-
Chalmers University of Technology
-
University of Gothenburg