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Insulin-Like Growth...
Insulin-Like Growth Factor-1 Supplementation Promotes Brain Maturation in Preterm Pigs
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- Christiansen, Line I. (author)
- University of Copenhagen
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- Holmqvist, Bo (author)
- ImaGene-iT
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- Pan, Xiaoyu (author)
- University of Copenhagen
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- Holgersen, Kristine (author)
- University of Copenhagen
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- Lindholm, Sandy E.H. (author)
- University of Copenhagen
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- Henriksen, Nicole L. (author)
- University of Copenhagen
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- Burrin, Douglas G. (author)
- Texas Children’s Hospital
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- Ley, David (author)
- Lund University,Lunds universitet,Pediatrik, Lund,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Neonatologi,Forskargrupper vid Lunds universitet,LTH profilområde: Avancerade ljuskällor,LTH profilområden,Lunds Tekniska Högskola,LU profilområde: Ljus och material,Lunds universitets profilområden,Paediatrics (Lund),Section V,Department of Clinical Sciences, Lund,Faculty of Medicine,Neonatology,Lund University Research Groups,LTH Profile Area: Photon Science and Technology,LTH Profile areas,Faculty of Engineering, LTH,LU Profile Area: Light and Materials,Lund University Profile areas
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- Thymann, Thomas (author)
- University of Copenhagen
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- Sangild, Per Torp (author)
- University of Copenhagen,Copenhagen University Hospital,Odense University Hospital
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- Pankratova, Stanislava (author)
- University of Copenhagen
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(creator_code:org_t)
- 2023
- 2023
- English.
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In: eNeuro. - 2373-2822. ; 10:4
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Abstract
Subject headings
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- Very preterm infants show low levels of insulin-like growth factor-1 (IGF-1), which is associated with postnatal growth restriction and poor neurologic outcomes. It remains unknown whether supplemental IGF-1 may stimulate neurode-velopment in preterm neonates. Using cesarean-delivered preterm pigs as a model of preterm infants, we investi-gated the effects of supplemental IGF-1 on motor function and on regional and cellular brain development. Pigs were treated with 2.25 mg/kg/d recombinant human IGF-1/IGF binding protein-3 complex from birth until day 5 or 9 before the collection of brain samples for quantitative immunohistochemistry (IHC), RNA sequencing, and quantitative PCR analyses. Brain protein synthesis was measured using in vivo labeling with [2H5] phenylalanine. We showed that the IGF-1 receptor was widely distributed in the brain and largely coexisted with immature neurons. Region-spe-cific quantification of IHC labeling showed that IGF-1 treatment promoted neuronal differentiation, increased subcorti-cal myelination, and attenuated synaptogenesis in a region-dependent and time-dependent manner. The expression levels of genes involved in neuronal and oligodendrocyte maturation, and angiogenic and transport functions were al-tered, reflecting enhanced brain maturation in response to IGF-1 treatment. Cerebellar protein synthesis was increased by 19% at day 5 and 14% at day 9 after IGF-1 treatment. Treatment had no effect on Iba1+ microglia or regional brain weights and did not affect motor development or the expression of genes related to IGF-1 signaling. In conclusion, the data show that supplemental IGF-1 promotes brain maturation in newborn preterm pigs. The results provide further support for IGF-1 supplementation therapy in the early postnatal period in preterm infants.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- cortex
- developing brain
- hippocampus
- IGF-1
- IGF1R
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Christiansen, Li ...
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Holmqvist, Bo
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Pan, Xiaoyu
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Holgersen, Krist ...
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Lindholm, Sandy ...
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Henriksen, Nicol ...
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show more...
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Burrin, Douglas ...
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Ley, David
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Thymann, Thomas
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Sangild, Per Tor ...
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Pankratova, Stan ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
- Articles in the publication
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eNeuro
- By the university
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Lund University