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Actin retrograde fl...
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Swaminathan, VinayNational Heart Lung and Blood Institute,Marine Biological Laboratory
(author)
Actin retrograde flow actively aligns and orients ligand-engaged integrins in focal adhesions
- Article/chapterEnglish2017
Publisher, publication year, extent ...
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2017-10-03
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Proceedings of the National Academy of Sciences,2017
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6 s.
Numbers
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LIBRIS-ID:oai:lup.lub.lu.se:a6fe995c-14fb-477f-91b1-d615262bebc3
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https://lup.lub.lu.se/record/a6fe995c-14fb-477f-91b1-d615262bebc3URI
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https://doi.org/10.1073/pnas.1701136114DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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Integrins are transmembrane receptors that, upon activation, bind extracellular ligands and link them to the actin filament (F-actin) cytoskeleton to mediate cell adhesion and migration. Cytoskeletal forces in migrating cells generated by polymerization- or contractility-driven “retrograde flow” of F-actin from the cell leading edge have been hypothesized to mediate integrin activation for ligand binding. This predicts that these forces should align and orient activated, ligand-bound integrins at the leading edge. Here, polarization-sensitive fluorescence microscopy of GFP-αVβ3 integrins in fibroblasts shows that integrins are coaligned in a specific orientation within focal adhesions (FAs) in a manner dependent on binding immobilized ligand and a talin-mediated linkage to the F-actin cytoskeleton. These findings, together with Rosetta modeling, suggest that integrins in FA are coaligned and may be highly tilted by cytoskeletal forces. Thus, the F-actin cytoskeleton sculpts an anisotropic molecular scaffold in FAs, and this feature may underlie the ability of migrating cells to sense directional extracellular cues.
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Kalappurakkal, Joseph MathewNational Centre for Biological Sciences,Marine Biological Laboratory
(author)
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Mehta, Shalin B.Marine Biological Laboratory
(author)
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Nordenfelt, PontusLund University,Lunds universitet,epIgG,Forskargrupper vid Lunds universitet,Lund University Research Groups,Harvard University,Boston Children's Hospital,Marine Biological Laboratory(Swepub:lu)med-pnl
(author)
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Moore, Travis I.Boston Children's Hospital,Harvard University,Marine Biological Laboratory
(author)
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Koga, NobuyasuInstitute for Molecular Science,University of Washington
(author)
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Baker, David A.University of Washington
(author)
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Oldenbourg, RudolfMarine Biological Laboratory
(author)
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Tani, TomomiMarine Biological Laboratory
(author)
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Mayor, SatyajitMarine Biological Laboratory,National Centre for Biological Sciences
(author)
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Springer, Timothy A.Marine Biological Laboratory,Boston Children's Hospital,Harvard University
(author)
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Waterman, Clare M.Marine Biological Laboratory,National Heart Lung and Blood Institute
(author)
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National Heart Lung and Blood InstituteMarine Biological Laboratory
(creator_code:org_t)
Related titles
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In:Proceedings of the National Academy of Sciences of the United States of America: Proceedings of the National Academy of Sciences114:40, s. 10648-106530027-8424
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In:Proceedings of the National Academy of Sciences: Proceedings of the National Academy of Sciences114:40, s. 10648-106531091-6490
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Swaminathan, Vin ...
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Kalappurakkal, J ...
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Mehta, Shalin B.
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Nordenfelt, Pont ...
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Moore, Travis I.
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Koga, Nobuyasu
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Baker, David A.
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Oldenbourg, Rudo ...
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Tani, Tomomi
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Mayor, Satyajit
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Springer, Timoth ...
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Waterman, Clare ...
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