Assignment of gene for coagulation factor V to chromosome 1 in man and to chromosome 13 in rat

• Two different factor V cDNA fragments were used as hydridization probes in the chromosomal assignment of the human and rat factor V genes. A 1.6-kb EcoRI fragment was used as a hybridization probe to analyze a panel of human-rodent somatic cell hybrids. Cosegregation of factor V specific DNA restriction fragments with human chromosome 1 was observed. In addition, a panel of rat-mouse somatic cell hybrids was analyzed with another human factor V cDNA probe to localize the gene for rat coagulation factor V. In the rat, the gene for coagulation factor V was found to be located in chromosome 13. This is the first gene in the rat to be localized to chromosome 13.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Läkemedelskemi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Medicinal Chemistry (hsv//eng)

Keyword

DNA Probes

Molecular

Cloning

Pair 1

Human

*Chromosomes

*Chromosome Mapping

Animals

Southern

Blotting

Factor V/*genetics

Humans

Hybrid Cells

Nucleic Acid Hybridization

Rats

Research Support

Non-U.S. Gov't

Publication and Content Type

art (subject category)

ref (subject category)