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Regulation of alter...
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MacKinnon, Alison C
(author)
Regulation of alternative macrophage activation by galectin-3
- Article/chapterEnglish2008
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LIBRIS-ID:oai:lup.lub.lu.se:aae7bacc-eb05-4f63-8755-cbe03579f9a8
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https://lup.lub.lu.se/record/1198625URI
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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Alternative macrophage activation is implicated in diverse disease pathologies such as asthma, organ fibrosis, and granulomatous diseases, but the mechanisms underlying macrophage programming are not fully understood. Galectin-3 is a carbohydrate-binding lectin present on macrophages. We show that disruption of the galectin-3 gene in 129sv mice specifically restrains IL-4/IL-13-induced alternative macrophage activation in bone marrow-derived macrophages in vitro and in resident lung and recruited peritoneal macrophages in vivo without affecting IFN-gamma/LPS-induced classical activation or IL-10-induced deactivation. IL-4-mediated alternative macrophage activation is inhibited by siRNA-targeted deletion of galectin-3 or its membrane receptor CD98 and by inhibition of PI3K. Increased galectin-3 expression and secretion is a feature of alternative macrophage activation. IL-4 stimulates galectin-3 expression and release in parallel with other phenotypic markers of alternative macrophage activation. By contrast, classical macrophage activation with LPS inhibits galectin-3 expression and release. Galectin-3 binds to CD98, and exogenous galectin-3 or cross-linking CD98 with the mAb 4F2 stimulates PI3K activation and alternative activation. IL-4-induced alternative activation is blocked by bis-(3-deoxy-3-(3-methoxybenzamido)-beta-D-galactopyranosyl) sulfane, a specific inhibitor of extracellular galectin-3 carbohydrate binding. These results demonstrate that a galectin-3 feedback loop drives alternative macrophage activation. Pharmacological modulation of galectin-3 function represents a novel therapeutic strategy in pathologies associated with alternatively activated macrophages.
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Farnworth, Sarah L
(author)
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Hodkinson, Philip S
(author)
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Henderson, Neil C
(author)
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Atkinson, Kirsten M
(author)
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Leffler, HakonLund University,Lunds universitet,Avdelningen för mikrobiologi, immunologi och glykobiologi - MIG,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Microbiology, Immunology and Glycobiology - MIG,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)mmb-hle
(author)
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Nilsson, UlfLund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)ok2-uni
(author)
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Haslett, Christopher
(author)
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Forbes, Stuart J
(author)
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Sethi, Tariq
(author)
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Avdelningen för mikrobiologi, immunologi och glykobiologi - MIGInstitutionen för laboratoriemedicin
(creator_code:org_t)
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In:Journal of Immunology180:4, s. 2650-26581550-6606
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Hodkinson, Phili ...
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Henderson, Neil ...
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Atkinson, Kirste ...
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Leffler, Hakon
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Nilsson, Ulf
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Haslett, Christo ...
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Forbes, Stuart J
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Sethi, Tariq
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- MEDICAL AND HEALTH SCIENCES
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Lund University