Search: onr:"swepub:oai:lup.lub.lu.se:ab856ed0-a683-469e-bead-cf78b07b571f" >
Leukemic stem cell ...
-
Thielen, NoortjeAmsterdam UMC - Vrije Universiteit Amsterdam
(author)
Leukemic stem cell quantification in newly diagnosed chronic myeloid leukemia patients predicts response to nilotinib therapy
- Article/chapterEnglish2016
Publisher, publication year, extent ...
Numbers
-
LIBRIS-ID:oai:lup.lub.lu.se:ab856ed0-a683-469e-bead-cf78b07b571f
-
https://lup.lub.lu.se/record/ab856ed0-a683-469e-bead-cf78b07b571fURI
-
https://doi.org/10.1158/1078-0432.CCR-15-2791DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:art swepub-publicationtype
-
Subject category:ref swepub-contenttype
Notes
-
PURPOSE: Leukemic stem cells (LSCs) may harbor important resistance to tyrosine kinase inhibitors in chronic myeloid leukemia (CML). We identified Philadelphia chromosome (Ph) positive CD34+CD38- bone marrow cells (here denoted LSCs) and addressed their response-predictive value in CML patients (n=48) subjected to nilotinib in the ENEST1st trial (NCT01061177).EXPERIMENTAL DESIGN: Two flow cytometry-based cell sorting methods were employed with multiparameter-directed CD45- (MPFC) and BCR-ABL1 probe-linked (FISH) identification of Ph-positive cells, respectively.RESULTS: We observed a positive correlation between the proportion of LSCs at diagnosis and established prognostic markers (blast count, spleen size, Sokal score, hemoglobin). Conversely, a high LSC burden predicted for an inferior molecular response at 3 (MPFC, FISH), 6 (MPFC), 9 (FISH) and 15 months (FISH). During nilotinib therapy, the proportion of LSCs decreased rapidly. At 3 months, a median of only 0.3% LSCs remained among CD34+CD38- cells, and in 33% of the patients the LSC clone was not detectable anymore (FISH). The response kinetics was similar in LSC fractions as it was in the progenitor and unseparated bone marrow cell fractions.CONCLUSION: The proportion of LSCs at diagnosis, as analyzed by two independent methodologies, reflects the biology of the disease and appeared as a prognostic and response-predictive marker in CML patients subjected to first-line nilotinib therapy.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Richter, JohanLund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine,Skåne University Hospital(Swepub:lu)med-jri
(author)
-
Baldauf, MatthiasMedical University of Innsbruck
(author)
-
Barbany, GiselaKarolinska Institute
(author)
-
Fioretos, ThoasLund University,Lunds universitet,Avdelningen för klinisk genetik,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Genetics,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)kgen-tfi
(author)
-
Giles, FrancisNorthwestern University
(author)
-
Gjertsen, Bjørn ToreUniversity of Bergen
(author)
-
Hochhaus, AndreasUniversitätsklinikum Jena
(author)
-
Schuurhuis, Gerrit JanAmsterdam UMC - Vrije Universiteit Amsterdam
(author)
-
Sopper, SieghartMedical University of Innsbruck
(author)
-
Stenke, LeifKarolinska University Hospital
(author)
-
Thunberg, SarahKarolinska University Hospital
(author)
-
Wolf, DominikUniversity Hospital Bonn
(author)
-
Ossenkoppele, GertAmsterdam UMC - Vrije Universiteit Amsterdam
(author)
-
Porkka, KimmoUniversity of Helsinki
(author)
-
Janssen, JeroenAmsterdam UMC - Vrije Universiteit Amsterdam
(author)
-
Mustjoki, SatuUniversity of Helsinki
(author)
-
Amsterdam UMC - Vrije Universiteit AmsterdamAvdelningen för molekylärmedicin och genterapi
(creator_code:org_t)
Related titles
-
In:Clinical Cancer Research22:16, s. 4030-40381078-0432
Internet link
Find in a library
To the university's database
- By the author/editor
-
Thielen, Noortje
-
Richter, Johan
-
Baldauf, Matthia ...
-
Barbany, Gisela
-
Fioretos, Thoas
-
Giles, Francis
-
show more...
-
Gjertsen, Bjørn ...
-
Hochhaus, Andrea ...
-
Schuurhuis, Gerr ...
-
Sopper, Sieghart
-
Stenke, Leif
-
Thunberg, Sarah
-
Wolf, Dominik
-
Ossenkoppele, Ge ...
-
Porkka, Kimmo
-
Janssen, Jeroen
-
Mustjoki, Satu
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
- Articles in the publication
-
Clinical Cancer ...
- By the university
-
Lund University