Search: onr:"swepub:oai:lup.lub.lu.se:affef12b-cad5-4d6e-9d43-0ecbeb684d7a" > Actin filament guid...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 05098naa a2200409 4500 | |
001 | oai:lup.lub.lu.se:affef12b-cad5-4d6e-9d43-0ecbeb684d7a | |
003 | SwePub | |
008 | 160401s2006 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/3983442 URI |
024 | 7 | a https://doi.org/10.1021/la060854i2 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Sundberg, Mark4 aut |
245 | 1 0 | a Actin filament guidance on a chip: Toward high-throughput assays and lab-on-a-chip applications |
264 | c 2006-07-22 | |
264 | 1 | b American Chemical Society (ACS),c 2006 |
520 | a Biological molecular motors that are constrained so that function is effectively limited to predefined nanosized tracks may be used as molecular shuttles in nanotechnological applications. For these applications and in high-throughput functional assays (e. g., drug screening), it is important that the motors propel their cytoskeletal filaments unidirectionally along the tracks with a minimal number of escape events. We here analyze the requirements for achieving this for actin filaments that are propelled by myosin II motor fragments (heavy meromyosin; HMM). First, we tested the guidance of HMM-propelled actin filaments along chemically defined borders. Here, trimethylchlorosilane (TMCS)-derivatized areas with high-quality HMM function were surrounded by SiO2 domains where HMM did not bind actin. Guidance along the TMCS-SiO2 border was almost 100% for filament approach angles between 0 and 20 degrees but only about 10% at approach angles near 90 degrees. A model (Clemmens, J.; Hess, H.; Lipscomb, R.; Hanein, Y.; Bohringer, K. F.; Matzke, C. M.; Bachand, G. D.; Bunker, B. C.; Vogel, V. Langmuir 2003, 19, 10967-10974) accounted for essential aspects of the data and also correctly predicted a more efficient guidance of actin filaments than previously shown for kinesin- propelled microtubules. Despite the efficient guidance at low approach angles, nanosized (< 700 nm wide) TMCS tracks surrounded by SiO2 were not effective in guiding actin filaments. Neither was there complete guidance along nanosized tracks that were surrounded by topographical barriers (walls and roof partially covering the track) unless there was also chemically based selectivity between the tracks and surroundings. In the latter case, with dually defined tracks, there was close to 100% guidance. A combined experimental and theoretical analysis, using tracks of the latter type, suggested that a track width of less than about 200-300 nm is sufficient at a high HMM surface density to achieve unidirectional sliding of actin filaments. In accord with these results, we demonstrate the long- term trapping of actin filaments on a closed-loop track (width < 250 nm). The results are discussed in relation to lab-on-a-chip applications and nanotechnology-assisted assays of actomyosin function. | |
650 | 7 | a NATURVETENSKAPx Fysikx Den kondenserade materiens fysik0 (SwePub)103042 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Physical Sciencesx Condensed Matter Physics0 (SwePub)103042 hsv//eng |
700 | 1 | a Bunk, Richardu Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)ftf-rbu |
700 | 1 | a Albet-Torres, Nuria4 aut |
700 | 1 | a Kvennefors, Andersu Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)ftf-ark |
700 | 1 | a Persson, Fredrik4 aut |
700 | 1 | a Montelius, Larsu Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)ftf-lmo |
700 | 1 | a Nicholls, Ian A.4 aut |
700 | 1 | a Ghatnekar-Nilsson, Sarau Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)insek-sn |
700 | 1 | a Omling, Päru Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)ftf-pom |
700 | 1 | a Tagerud, Sven4 aut |
700 | 1 | a Mansson, Alf4 aut |
710 | 2 | a Fasta tillståndets fysikb Fysiska institutionen4 org |
773 | 0 | t Langmuird : American Chemical Society (ACS)g 22:17, s. 7286-7295q 22:17<7286-7295x 0743-7463x 1520-5827 |
856 | 4 | u http://dx.doi.org/10.1021/la060854iy FULLTEXT |
856 | 4 8 | u https://lup.lub.lu.se/record/398344 |
856 | 4 8 | u https://doi.org/10.1021/la060854i |
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.