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Characterization of...
Characterization of GYP*Mur and novel GYP*Bun-like hybrids in Thai blood donors reveals a qualitatively altered s antigen
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- Jongruamklang, Philaiphon (author)
- Lund University,Lunds universitet,Transfusionsmedicin,Forskargrupper vid Lunds universitet,Transfusion Medicine,Lund University Research Groups,University of Phayao
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- Grimsley, Shane (author)
- International Blood Group Reference Laboratory
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- Thornton, Nicole (author)
- International Blood Group Reference Laboratory
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- Robb, Janine (author)
- Quotient, Edinburgh
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- Olsson, Martin L. (author)
- Lund University,Lunds universitet,Transfusionsmedicin,Forskargrupper vid Lunds universitet,Transfusion Medicine,Lund University Research Groups
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- Storry, Jill R. (author)
- Lund University,Lunds universitet,Transfusionsmedicin,Forskargrupper vid Lunds universitet,Transfusion Medicine,Lund University Research Groups
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(creator_code:org_t)
- 2020-03-23
- 2020
- English 6 s.
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In: Vox Sanguinis. - : Wiley. - 0042-9007 .- 1423-0410. ; 115:5, s. 472-477
- Related links:
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http://dx.doi.org/10... (free)
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Subject headings
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- Background and objectives: The Mi(a+) GP(B-A-B) hybrid phenotypes occur with a prevalence of 2%–23% across Southeast Asia. While the s antigen is alleged to be altered, no evidence for specific variants is known. Screening using a monoclonal IgM anti-s mistyped six S‒s+ RBC units as S‒s‒. Further, alloanti-s was identified in an S+s+ patient. Our objective was to investigate the s antigen further. Materials and methods: DNA from 63 Thai blood donor samples PCR-positive for a GYP(B-A-B) hybrid was sequenced with primers spanning GYPB exons 3–4. Flow cytometry was used for semiquantitative analysis of s expression and correlated with the glycophorin genotype. Results: DNA sequencing showed that GYP*Mur was carried by 56/63 samples (88·9%) of which 5/56 lacked normal GYPB: three of these were GYP*Mur homozygotes, one was a compound heterozygote carrying GYP*Mur and a GYP*Bun-like allele (designated GYP*Thai), and the fifth sample carried GYP*Mur and another GYP*Bun-like allele. Seven samples (7/63) were GYP*Thai heterozygotes. IgM monoclonal anti-s (P3BER) did not react with the s antigen carried by GP.Mur or GP.Bun, whereas two IgG anti-s showed enhanced reactivity. Conclusions: We confirmed that GYP*Mur is the most frequent variant in Thai blood donors and also identified GYP*Thai with a frequency of 1·1%. We showed that s antigen on Mi(a+) GP(B-A-B) hybrids is qualitatively altered and should be considered when selecting reagents for phenotyping where such hybrids are prevalent, endemically and in blood centres worldwide.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
Keyword
- blood group antigen
- glycophorin
- GP.Mur
Publication and Content Type
- art (subject category)
- ref (subject category)
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