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Generation of the h...
Generation of the human pluripotent stem-cell-derived astrocyte model with forebrain identity
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- Peteri, Ulla Kaisa (author)
- University of Helsinki
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- Pitkonen, Juho (author)
- University of Helsinki
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- Utami, Kagistia Hana (author)
- Agency for Science, Technology and Research (A*STAR)
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- Paavola, Jere (author)
- Minerva Foundation Institute for Medical Research
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- Roybon, Laurent (author)
- Lund University,Lunds universitet,Stamcellslaboratoriet för sjukdomsmodellering i det centrala nervsystemet,Forskargrupper vid Lunds universitet,IPSC Laboratory for CNS Disease Modeling,Lund University Research Groups
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- Pouladi, Mahmoud A. (author)
- Agency for Science, Technology and Research (A*STAR)
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- Castrén, Maija L. (author)
- University of Helsinki
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(creator_code:org_t)
- 2021-02-09
- 2021
- English 14 s.
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In: Brain Sciences. - : MDPI AG. - 2076-3425. ; 11:2
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Abstract
Subject headings
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- Astrocytes form functionally and morphologically distinct populations of cells with brain-region-specific properties. Human pluripotent stem cells (hPSCs) offer possibilities to generate astroglia for studies investigating mechanisms governing the emergence of astrocytic diversity. We established a method to generate human astrocytes from hPSCs with forebrain patterning and final specification with ciliary neurotrophic factor (CNTF). Transcriptome profiling and gene enrichment analysis monitored the sequential expression of genes determining astrocyte differentiation and confirmed activation of forebrain differentiation pathways at Day 30 (D30) and D60 of differentiation in vitro. More than 90% of astrocytes aged D95 in vitro co-expressed the astrocytic markers glial fibrillary acidic protein (GFAP) and S100β. Intracellular calcium responses to ATP indicated differentiation of the functional astrocyte population with constitutive monocyte chemoattractant protein-1 (MCP-1/CCL2) and tissue inhibitor of metalloproteinases-2 (TIMP-2) expression. The method was reproducible across several hPSC lines, and the data demonstrated the usefulness of forebrain astrocyte modeling in research investigating forebrain pathology.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Astrocytes
- Differentiation
- Fragile X syndrome
- Patterning
- Pluripotent stem cells
Publication and Content Type
- art (subject category)
- ref (subject category)
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