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GSK-3β-induced Tau ...
GSK-3β-induced Tau pathology drives hippocampal neuronal cell death in Huntington's disease : involvement of astrocyte-neuron interactions
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- L'Episcopo, F (author)
- OASI Institute for Research and Care on Mental Retardation and Brain Aging (IRCCS),University of Catania
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- Drouin-Ouellet, J (author)
- Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Developmental and Regenerative Neurobiology,Lund University Research Groups,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine
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- Tirolo, C (author)
- OASI Institute for Research and Care on Mental Retardation and Brain Aging (IRCCS)
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- Pulvirenti, A (author)
- University of Catania
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- Giugno, R (author)
- University of Catania
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- Testa, N (author)
- OASI Institute for Research and Care on Mental Retardation and Brain Aging (IRCCS)
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- Caniglia, S (author)
- OASI Institute for Research and Care on Mental Retardation and Brain Aging (IRCCS)
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- Serapide, M F (author)
- University of Catania
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- Cisbani, G (author)
- Centre Hospitalier de l'Universite Laval (CHUL)
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- Barker, R A (author)
- Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Developmental and Regenerative Neurobiology,Lund University Research Groups,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine,University of Cambridge
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- Cicchetti, F (author)
- Centre Hospitalier de l'Universite Laval (CHUL),Laval University
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- Marchetti, B (author)
- OASI Institute for Research and Care on Mental Retardation and Brain Aging (IRCCS),University of Catania
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(creator_code:org_t)
- 2016-04-28
- 2016
- English.
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In: Cell Death and Disease. - : Springer Science and Business Media LLC. - 2041-4889. ; 7, s. 1-14
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Abstract
Subject headings
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- Glycogen synthase kinase-3β (GSK-3β) has emerged as a critical factor in several pathways involved in hippocampal neuronal maintenance and function. In Huntington's disease (HD), there are early hippocampal deficits both in patients and transgenic mouse models, which prompted us to investigate whether disease-specific changes in GSK-3β expression may underlie these abnormalities. Thirty-three postmortem hippocampal samples from HD patients (neuropathological grades 2-4) and age- and sex-matched normal control cases were analyzed using real-time quantitative reverse transcription PCRs (qPCRs) and immunohistochemistry. In vitro and in vivo studies looking at hippocampal pathology and GSK-3β were also undertaken in transgenic R6/2 and wild-type mice. We identified a disease and stage-dependent upregulation of GSK-3β mRNA and protein levels in the HD hippocampus, with the active isoform pGSK-3β-Tyr(216) being strongly expressed in dentate gyrus (DG) neurons and astrocytes at a time when phosphorylation of Tau at the AT8 epitope was also present in these same neurons. This upregulation of pGSK-3β-Tyr(216) was also found in the R6/2 hippocampus in vivo and linked to the increased vulnerability of primary hippocampal neurons in vitro. In addition, the increased expression of GSK-3β in the astrocytes of R6/2 mice appeared to be the main driver of Tau phosphorylation and caspase3 activation-induced neuronal death, at least in part via an exacerbated production of major proinflammatory mediators. This stage-dependent overactivation of GSK-3β in HD-affected hippocampal neurons and astrocytes therefore points to GSK-3β as being a critical factor in the pathological development of this condition. As such, therapeutic targeting of this pathway may help ameliorate neuronal dysfunction in HD.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- Journal Article
- Research Support, Non-U.S. Gov't
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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L'Episcopo, F
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Drouin-Ouellet, ...
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Tirolo, C
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Pulvirenti, A
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Giugno, R
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Testa, N
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show more...
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Caniglia, S
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Serapide, M F
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Cisbani, G
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Barker, R A
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Cicchetti, F
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Marchetti, B
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
- Articles in the publication
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Cell Death and D ...
- By the university
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Lund University