A new approach to detection of antigen-antibody complexes by microcalorimetric measurements of heat production in blood cells

• Using batch microcalorimeters of the thermopile conduction type, heat production was measured in human blood in the presence of defined antigens and their specific antibodies. A large increase in heat production, reflecting increased metabolic activity in the blood cells, was found when antigens and specific antisera at certain antigen-antibody proportions were mixed with the blood. The reproducibility and specificity of the method were found to be very good. The present results indicate that calorimetry may be a useful technique for detection of antigen-antibody complexes.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Hematologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Hematology (hsv//eng)

Publication and Content Type

art (subject category)

ref (subject category)