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Modeling neural tub...
Modeling neural tube development by differentiation of human embryonic stem cells in a microfluidic WNT gradient
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- Rifes, Pedro (author)
- University of Copenhagen
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- Isaksson, Marc (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Department of Experimental Medical Science,Faculty of Medicine
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- Rathore, Gaurav Singh (author)
- University of Copenhagen
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- Aldrin-Kirk, Patrick (author)
- Lund University,Lunds universitet,University of Copenhagen
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- Møller, Oliver Knights (author)
- University of Copenhagen
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- Barzaghi, Guido (author)
- University of Copenhagen
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- Lee, Julie (author)
- University of Copenhagen
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- Egerod, Kristoffer Lihme (author)
- University of Copenhagen
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- Rausch, Dylan M. (author)
- University of Copenhagen
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- Parmar, Malin (author)
- Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups
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- Pers, Tune H. (author)
- University of Copenhagen
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- Laurell, Thomas (author)
- Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Other operations, LTH,Faculty of Engineering, LTH,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,LUCC: Lund University Cancer Centre,Other Strong Research Environments
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- Kirkeby, Agnete (author)
- Lund University,Lunds universitet,Human neural utvecklingsbiologi,Forskargrupper vid Lunds universitet,WCMM- Wallenberg center för molekylär medicinsk forskning,Medicinska fakulteten,Human Neural Developmental Biology,Lund University Research Groups,WCMM-Wallenberg Centre for Molecular Medicine,Faculty of Medicine,University of Copenhagen
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(creator_code:org_t)
- 2020-05-25
- 2020
- English 9 s.
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In: Nature Biotechnology. - : Springer Science and Business Media LLC. - 1087-0156 .- 1546-1696. ; 38:11, s. 1265-1273
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http://dx.doi.org/10...
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Abstract
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- The study of brain development in humans is limited by the lack of tissue samples and suitable in vitro models. Here, we model early human neural tube development using human embryonic stem cells cultured in a microfluidic device. The approach, named microfluidic-controlled stem cell regionalization (MiSTR), exposes pluripotent stem cells to signaling gradients that mimic developmental patterning. Using a WNT-activating gradient, we generated a neural tissue exhibiting progressive caudalization from forebrain to midbrain to hindbrain, including formation of isthmic organizer characteristics. Single-cell transcriptomics revealed that rostro-caudal organization was already established at 24 h of differentiation, and that the first markers of a neural-specific transcription program emerged in the rostral cells at 48 h. The transcriptomic hallmarks of rostro-caudal organization recapitulated gene expression patterns of the early rostro-caudal neural plate in mouse embryos. Thus, MiSTR will facilitate research on the factors and processes underlying rostro-caudal neural tube patterning.
Subject headings
- NATURVETENSKAP -- Biologi -- Utvecklingsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Developmental Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Rifes, Pedro
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Isaksson, Marc
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Rathore, Gaurav ...
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Aldrin-Kirk, Pat ...
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Møller, Oliver K ...
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Barzaghi, Guido
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show more...
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Lee, Julie
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Egerod, Kristoff ...
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Rausch, Dylan M.
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Parmar, Malin
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Pers, Tune H.
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Laurell, Thomas
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Kirkeby, Agnete
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Developmental Bi ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Nature Biotechno ...
- By the university
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Lund University